GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE268042 Query DataSets for GSE268042
Status Public on May 30, 2024
Title Gene expression profiling of Elastin (ELN) knock-down in human fibroblast MRC5
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The ELN gene encodes tropoelastin which is used to generate elastic fibers that insure proper tissue elasticity. Decreased amounts of elastic fibers and/or accumulation of bioactive products of their cleavage, named elastokines, are thought to contribute to aging. Cellular senescence, characterized by a stable proliferation arrest and by the senescence-associated secretory phenotype (SASP), increases with aging. Here, we identified that decrease in ELN either by siRNA in normal human fibroblasts results in premature senescence. Surprisingly this effect is independent of elastic fiber degradation or elastokines production, but it relies on the rapid increase in HMOX1 after ELN downregulation. These results unveil a role for ELN in protecting cells from cellular senescence through a non-canonical mechanism.
Overall design Normal human fibroblast cells (MRC5) were transfected with siRNA against Elastin gene (siELN) or with scramble siRNA (siCTRL). Gene expression profiling was performed 1 day or 4 days after transfection. Three independent replicates have been performed.
Contributor(s) Flaman J, Czarnecka-Herok J, Martin N, Bernard D
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 21, 2024
Last update date May 30, 2024
Contact name jean-michel flaman
Phone +33687477812
Organization name Inserm 1052
Department CRCL
Street address 28 rue Laennec
City Lyon
ZIP/Postal code 69373
Country France
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (9)
GSM8284803 MRC5_siCTRL_R1
GSM8284804 MRC5_siCTRL_R2
GSM8284805 MRC5_siCTRL_R3
BioProject PRJNA1114092

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE268042_RAW.tar 19.2 Mb (http)(custom) TAR (of TXT)

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap