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Series GSE268447 Query DataSets for GSE268447
Status Public on Jun 02, 2024
Title lncRNA-ZFAS1, an emerging gate-keeper in DNA damage-dependent transcriptional regulation [OE CTD ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Numerous long noncoding RNAs (lncRNAs) are generated in response to external stimuli, but the scope and functions of such activity are not known. Here, we provide insight into how the transcription of lncRNAs are connected to DNA damage response by identifying a lncRNA ZFAS1, which is required for cell cycle arrest, transcription regulation and DNA repair. Mechanistically, ZFAS1 facilitates the changing hyperphosphorylated forms of the large subunit of RNAPII around transcription initiation sites by directly targeting the regulated genes. We revealed extensive transcription shutdown and concomitant stimulated engagement of RNAPII-Ser2P are crucial for repair and cell survival upon genotoxic stress. Finally, ZFAS1 knockout in mice dampened transcription-coupled nucleotide excision repair (TC-NER) and led to kidney dysplasia. Our study extends the understanding of lncRNAs in DNA damage repair (DDR) and implies a protective role of lncRNA against DDR-deficient developmental disorders.
 
Overall design Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for RNAPII CTD Ser2P and Ser5P in the MRC5 cells overexpressing human ZFAS1 with or without UV-C irradiation.
 
Contributor(s) Liu J, Lu Q, Zhu T, Fan Z, He N, Wang Y
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Submission date May 28, 2024
Last update date Jun 03, 2024
Contact name Yuming Wang
E-mail(s) wangyuming2006@hotmail.com
Phone 008602037103555
Organization name Guangzhou medical university
Department School of Basic Medical Science
Street address Room 512, Anatomy Building
City Guangzhou
State/province Guangdong
ZIP/Postal code 511436
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM8291469 MRC5_ZFAS1 overexpression, input
GSM8291470 MRC5_ZFAS1 overexpression, CTD Ser5P
GSM8291471 MRC5_ZFAS1 overexpression, CTD Ser2P
This SubSeries is part of SuperSeries:
GSE239617 lncRNA-ZFAS1, an emerging gate-keeper in DNA damage-dependent transcriptional regulation
Relations
BioProject PRJNA1117394

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE268447_RAW.tar 508.8 Mb (http)(custom) TAR (of BW, NARROWPEAK)
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Raw data are available in SRA

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