GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE268542 Query DataSets for GSE268542
Status Public on May 29, 2024
Title 10x single-cell RNAseq profiling of influenza Virus infected Human Lung Tissue Explants (HLTE)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary This study investigates the interactions between Influenza A virus (IAV) and lung tissue at the single-cell level using the 10x Genomics single-cell RNA sequencing (scRNAseq) platform. Lung tissue samples from a donor with COPD were infected with IAV, and both infected and uninfected samples were analyzed 24 hours post-infection. Single-cell suspensions from multiple infected and uninfected tissue pieces were pooled and analyzed to identify cell types and their responses. A total of 10 cell types were identified, including various airway epithelial cells and immune cells. Apoptotic T cells, characterized by high mitochondrial DNA content, were excluded from gene expression analyses. The presence of viral mRNA was used to indicate viral transcription activity, although distinguishing between intracellular synthesis and extracellular uptake of viral mRNA remains challenging.
Overall design Human Lung tissue explant from COPD patients were cultrured in in vitro . These tissue pieces were infected with influenz A virus for 24 hours . After infection, tissue was digested into single cell suspension.
Contributor(s) Pessler F, Geffers R, Sohail A
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 29, 2024
Last update date May 30, 2024
Contact name Frank Pessler
Phone 0511-220027-167
Organization name TWINCORE Centre for Experimental and Clinical Infection Reearch
Department Experimental Infection Research
Lab Research Group Biomarkers for Infectious Diseases
Street address Feodor-Lynen-Str. 7
City Hannover
State/province Lower Saxony
ZIP/Postal code 30625
Country Germany
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (2)
GSM8294047 Human lung tissue cells uninfected control
GSM8294048 Human lung tissue cells IAV infected
BioProject PRJNA1117768

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE268542_HuLu2023.RDS.gz 34.2 Mb (ftp)(http) RDS
GSE268542_RAW.tar 29.1 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap