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| Status |
Public on May 08, 2014 |
| Title |
Transcriptomic profile of Clostridium thermocellum ATCC27405 using switchgrass as carbon source |
| Organism |
Acetivibrio thermocellus ATCC 27405 |
| Experiment type |
Expression profiling by array
Expression profiling by genome tiling array
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| Summary |
Clostridium thermocellum is a Gram-positive, anaerobic, thermophilic bacterium that ferments cellulose into ethanol. It is a candidate industrial consolidated bioprocess (CBP) biocatalyst for lignocellulosic bioethanol production to produce bioethanol directly from cellulosic biomass. However, few transcriptomic studies have been reported so far for C. thermocellum using biomass as carbon source. In this study, samples were taken from exponential and stationary phases of C. thermocellum cells growing in MTC media with pretreated switchgrass as carbon source, and transcriptomic profiling change of C. thermocellum during different growth phase was investigated using both expression array and tiling array. This study will help the understanding of gene expression of C. thermocellum using cellulosic biomass as carbon source and the knowledge will facilitate future metabolic engineering effort for strain improvement.
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| Overall design |
[HX12 expression array]: A eleven array study using total RNA recovered from wild-type cultures of Clostridium thermocellum at different growth phase of T2 and T3 with switchgrass as carbon source. Two biological replicates used for each phase.
[3Plex tiling array]: A six array study using total RNA recovered from wild-type cultures of Clostridium thermocellum at different growth phase of T2 and T3 with switchgrass as carbon source. Two biological replicates used for each phase.
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| Contributor(s) |
Yang S, Brown SD, Dice L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 28, 2011 |
| Last update date |
May 09, 2014 |
| Contact name |
Shihui YANG |
| E-mail(s) |
Shihui.Yang@nrel.gov
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| Phone |
3033847825
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| Organization name |
National Renewable Energy Lab
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| Department |
National Bioenergy Center
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| Street address |
1617 Cole Blvd
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| City |
Golden |
| State/province |
CO |
| ZIP/Postal code |
80401 |
| Country |
USA |
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| Platforms (2) |
| GPL11186 |
Clostridium thermocellum ATCC 27405 9K (expr_HX12) |
| GPL11655 |
NimbleGen Clostridium_thermocellum_ATCC27405_Tiling_3Plex |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA136073 |
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