|
| Status |
Public on Aug 04, 2011 |
| Title |
Human peripheral blood CD4+ T cell subsets |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Cells were isolated from healthy human donors (n=2). Unstimulated cells. Cells were stained with CD4, CD45RA, CCR7 and CXCR7. Using flow cytometry, 4 CD4+ T cell populations were sorted: (1) Naïve (CD45RA+CCR7+CXCR5-), (2) Central memory (CD45RA-CCR7+CXCR5-), (3) Effector memory (CD45RA-CCR7-CXCR5-) and (4) CXCR5+ cells (CD45RA-CCR7-CXCR5+)
|
| |
|
| Overall design |
RNA were extracted from sorted cells and hybridized on Affymetix HU133Plus2 chips. Each population were repeated on different donors such that n=2.
|
| |
|
| Contributor(s) |
Ho E, Tangye SG |
| Citation(s) |
21471443 |
| |
| Submission date |
Jan 28, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Santi Suryani |
| E-mail(s) |
s.suryani@garvan.org.au
|
| Organization name |
Garvan Institute of Medical Research
|
| Department |
Immunology and Inflammation
|
| Street address |
384 Victoria Street, Darlinghurst
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (8)
|
| GSM663126 |
Human CD4+ central memory T cells (1) |
| GSM663127 |
Human CD4+ CXCR5+ T cells (1) |
| GSM663128 |
Human CD4+ effector memory T cells (1) |
|
| Relations |
| BioProject |
PRJNA136077 |
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