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| Status |
Public on Jul 10, 2024 |
| Title |
RNA-seq dataset for "Reelin marks cocaine-activated striatal ensembles, promotes neuronal excitability, and regulates cocaine reward" |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This dataset contains RNA-seq results from rat embryonic striatal neuronal cultures treated with CRISPRi machinery (dCas9-KRAB-MeCP2 fusion) with a CRISPR sgRNA targeting lacZ (a bacterial gene not found in the mammalian genome; used as a non-targeting control) or Reln.
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| Overall design |
This experiment contains 8 biological samples, each of which underwent RNA-seq on an Illumina NovaSeq6000 instrument. Samples were treated with CRISPRi machinery targeting lacZ (lacZ control; samples X1i, X2i, X3i, X4i), or Reln (samples X5i, X6i, X7i, X8i) . Forward and reverse paired-end reads are denoted as "R1" or "R2" in raw file names.
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| Contributor(s) |
Brida KL, Ianov L, Day JJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 07, 2024 |
| Last update date |
Jul 10, 2024 |
| Contact name |
Jeremy Jason Day |
| E-mail(s) |
jjday@uab.edu
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| Phone |
205-996-8960
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| Organization name |
UAB
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| Department |
Neurobiology
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| Lab |
Day
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| Street address |
1825 University Blvd
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| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1121282 |
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