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Series GSE26977 Query DataSets for GSE26977
Status Public on Dec 21, 2011
Title PhyK, PhyR, σT and σU regulons
Platform organism Caulobacter vibrioides CB15
Sample organism Caulobacter vibrioides NA1000
Experiment type Expression profiling by array
Summary The extracytoplasmic function sigma factor σT is the master regulator of general stress response in Caulobacter crescentus, and controls the expression of its paralogue σU. This work showed that PhyR and NepR act as positive and negative regulators, respectively, of σT expression and function. Biochemical data also indicated that NepR directly binds σT and the phosphorylated form of PhyR. We also demonstrated the essential role of the histidine kinase gene CC3474, here denominated phyK, for expression of σT-dependent genes and for resistance to stress conditions. Additionally, in vivo evidence of PhyK-dependent phosphorylation of PhyR is presented. This study also identified a conserved cysteine residue (C95) present in the periplasmic portion of PhyK that is crucial for the function of the protein. Furthermore, we showed that PhyK, PhyR and σT regulate the same set of genes and that σT is apparently responsible for the direct control of its complete regulon. In contrast, σU seems to have a very modest contribution for expression of a subset of σT-dependent genes. In conclusion, this work describes the mechanism involved in the control of general stress response in C. crescentus.
 
Overall design Five experimetal procedures, each of them performed in three replicates. A total of fifteen independent biological samples were used
 
Contributor(s) Lourenço RF, Kohler C, Gomes SL
Citation(s) 21564331
Submission date Jan 31, 2011
Last update date Apr 01, 2013
Contact name Rogerio Lourenco
E-mail(s) lourenco@iq.usp.br
Organization name Universidade de Sao Paulo
Department Bioquimica
Street address Av. Prof. Lineu Prestes, 748
City Sao Paulo
State/province Sao Paulo
ZIP/Postal code 05508-900
Country Brazil
 
Platforms (1)
GPL10469 MIT Agilent Caulobacter 45K Array
Samples (15)
GSM664118 ΔphyK x WT- rep1
GSM664119 ΔphyK x WT- rep2
GSM664120 ΔphyK x WT- rep3
Relations
BioProject PRJNA135921

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26977_RAW.tar 179.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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