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| Status |
Public on Feb 03, 2011 |
| Title |
Responses to ectoparasite salmon louse (Lepeophtheirus salmonis) in skin of Atlantic salmon |
| Platform organisms |
Oncorhynchus mykiss; Salmo salar |
| Sample organism |
Salmo salar |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling revealed rapid activation of immunity, both local and systemic, which however did not provide protection of fish against the parasite. Major changes of transcriptome responses wwere observed between days 5 and 10
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| Overall design |
Atlantic salmon was challenged with L. salmonis at the copepod stage. Skin, spleen, and head kidney were sampled from challenged and control fish at 1, 3, 5 dpi (corresponding to the copepod stage); 10 and 15 dpi (chalimus stage). A total of forty samples of spleen and skin form infected salmon (4 individuals from the 5 time points) were used for microarray analyses.. Test samples were labeled with Cy5 and hybridized to pooled control samples labeled with Cy3 from the same time-points. Competitive hybridization to the arrays was followed by washing, scanning, image analysis, and data analysis. Selected genes were analyzed with RT-qPCR.
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| Contributor(s) |
Krasnov A, Tadiso TM |
| Citation(s) |
21385383 |
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| Submission date |
Jan 31, 2011 |
| Last update date |
Mar 27, 2012 |
| Contact name |
Aleksei Krasnov |
| E-mail(s) |
aleksei.krasnov@nofima.no
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| Phone |
+47 97602165
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| Organization name |
Nofima AS
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| Department |
Fish Health
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| Street address |
Osloveien 1
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| City |
Aas |
| ZIP/Postal code |
1430 |
| Country |
Norway |
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| Platforms (1) |
| GPL10705 |
Agilent Salmon Immunity and Quality 44k custom array_SIQ3 |
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| Samples (20)
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| GSM664626 |
Skin, day 1, fish 4 |
| GSM664627 |
Skin, day 3, fish 1 |
| GSM664628 |
Skin, day 3, fish 2 |
| GSM664629 |
Skin, day 3, fish 3 |
| GSM664630 |
Skin, day 3, fish 4 |
| GSM664631 |
Skin, day 5, fish 1 |
| GSM664632 |
Skin, day 5, fish 2 |
| GSM664633 |
Skin, day 5, fish 3 |
| GSM664634 |
Skin, day 5, fish 4 |
| GSM664635 |
Skin, day 10, fish 1 |
| GSM664636 |
Skin, day 10, fish 2 |
| GSM664637 |
Skin, day 10, fish 3 |
| GSM664638 |
Skin, day 10, fish 4 |
| GSM664639 |
Skin, day 15, fish 1 |
| GSM664640 |
Skin, day 15, fish 2 |
| GSM664641 |
Skin, day 15, fish 3 |
| GSM664642 |
Skin, day 15, fish 4 |
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| This SubSeries is part of SuperSeries: |
| GSE27013 |
Responses to ectoparasite salmon louse (Lepeophtheirus salmonis) in Atlantic salmon |
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| Relations |
| BioProject |
PRJNA142029 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26981_RAW.tar |
66.7 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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