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Series GSE269962 Query DataSets for GSE269962
Status Public on Jul 15, 2024
Title IGF2BP3 promotes mRNA degradation through internal m7G modification [seq_Ribo]
Organism Homo sapiens
Experiment type Other
Summary Recent studies have suggested that mRNA internal m7G and its writer protein METTL1 are closely related to cell metabolism and cancer regulation. Here, we identify that IGF2BP family proteins IGF2BP1-3 can preferentially bind internal mRNA m7G. Such interactions, especially IGF2BP3 with m7G, could promote the degradation of m7G target transcripts in cancer cells. IGF2BP3 is more responsive to changes of the m7G modification, while IGF2BP1 prefers m6A to stabilize the bound transcripts. We also demonstrate that p53 transcript, TP53, is m7G-modified at its 3’UTR in cancer cells. In glioblastoma, the methylation level and the half lifetime of the modified transcript could be modulated by tuning IGF2BP3, or by site-specific targeting of m7G through a dCas13b-guided system, resulting in modulation of cancer progression and chemosensitivity.
 
Overall design Ribo-seq in HepG2 cells
 
Contributor(s) Liu C, Dou X, Zhao Y, He C
Citation(s) 39198433
Submission date Jun 15, 2024
Last update date Oct 14, 2024
Contact name Xiaoyang Dou
E-mail(s) xiaoyang.dou@sibcb.ac.cn
Organization name Center for Excellence in Molecular Cell Science, CAS
Street address 320 Yue Yang Road
City Shanghai
ZIP/Postal code 200031
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM8331545 HepG2 siControl Input rep1
GSM8331546 HepG2 siControl Input rep2
GSM8331547 HepG2 siControl Input rep3
This SubSeries is part of SuperSeries:
GSE241222 IGF2BP3 promotes mRNA degradation through internal m7G modification
Relations
BioProject PRJNA1124388

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Supplementary file Size Download File type/resource
GSE269962_RAW.tar 57.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA

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