|
| Status |
Public on Feb 11, 2011 |
| Title |
IGH analysis in pro-B cells with H3K4me3, H3K4me2, H3K9ac, H3K27me3, Pax5 and CTCF [ChIP-chip] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
VH-DJH recombination of the immunoglobulin heavy-chain (Igh) locus is temporally and spatially controlled during early B-cell development, and yet no regulatory elements other than the VH gene promoters have been identified throughout the entire 2.5-Mb VH gene cluster. Here we discovered novel regulatory sequences that are interspersed in the distal VH gene region. These conserved repeat elements were characterized by the presence of Pax5-dependent active chromatin, the binding of Pax5, E2A, CTCF and Rad21 as well as by Pax5-dependent antisense transcription in pro-B cells. The Pax5-activated intergenic repeat (PAIR) elements were no longer bound by Pax5 in pre-B and B cells consistent with the loss of antisense transcription, whereas E2A and CTCF interacted with PAIR elements throughout early B-cell development. The pro-B-cell-specific and Pax5-dependent activity of the PAIR elements suggests that they are involved in the regulation of distal VH-DJH recombination at the Igh locus.
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| |
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| Overall design |
Analysis of chromatin and TF binding in rag2-/- and pax5-/- rag2-/- pro-B cells. Chip-Chip with one experiment for each antibody, 12 samples.
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| |
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| Contributor(s) |
McManus S, Ebert A, Novatchkova M, Busslinger M, Jaritz M |
| Citation(s) |
21349430, 21552207 |
| |
| Submission date |
Feb 04, 2011 |
| Last update date |
Mar 30, 2012 |
| Contact name |
Meinrad Busslinger |
| E-mail(s) |
busslinger@imp.ac.at
|
| Phone |
00431-79730
|
| Organization name |
Instutute of Molecular Pathology
|
| Street address |
Dr. Bohrgasse 7
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platforms (1) |
|
| Samples (12)
|
| GSM668137 |
GenAu MB BCELL v5.0 H3K9ac Pax5-/- Rag2-/- |
| GSM668138 |
GenAu MB BCELL v5.0 H3K9ac Rag2-/- |
| GSM668139 |
GenAu MB BCELL v5.0 H3K4me2 Pax5-/- Rag2-/- |
| GSM668140 |
GenAu MB BCELL v5.0 H3K4me2 Rag2-/- |
| GSM668141 |
GenAu MB BCELL v5.0 H3K4me3 Pax5-/- Rag2-/- |
| GSM668142 |
GenAu MB BCELL v5.0 H3K4me3 Rag2-/- |
| GSM668143 |
GenAu MB BCELL v5.0 Pax5 Rag2-/- |
| GSM668144 |
GenAu MB BCELL v5.0 Ctcf Rag2-/- |
| GSM668145 |
GenAu MB BCELL v5.0 H3K27me3 Rag2-/- |
| GSM668146 |
GenAu MB BCELL v5.0 Ctcf Pax5-/- Rag2-/- |
| GSM668147 |
GenAu MB BCELL v5.0 H3K36me3 Rag2-/- |
| GSM668148 |
GenAu MB BCELL v5.0 Pax-bio Pax5bio/bio Rag2-/- |
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| This SubSeries is part of SuperSeries: |
| GSE27215 |
IGH analysis in pro-B cells with H3K4me3, H3K4me2, H3K9ac, H3K27me3, Pax5 and CTCF |
|
| Relations |
| BioProject |
PRJNA141951 |
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