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Series GSE27400 Query DataSets for GSE27400
Status Public on Dec 31, 2022
Title Comparative gene expression in mature egg cell, fertilized egg cell (1-1.5 hap) and fertilized zygote (2.5-3 hap)
Platform organism Oryza sativa
Sample organism Oryza sativa Japonica Group
Experiment type Expression profiling by array
Summary Egg cells represent the female partner that fuses with the sperm cell during fertilization in all multi-cellular eukaryotic organisms—and, in flowering plants, is a founder of the embryo. We examined the transcriptome of Oryza sativa ssp. japonica (cv Katy) using the Affymetrix 57K rice genome GeneChip to provide an overview of genes activated in the maternal gamete, transcripts transmitted by the paternal genome, and changes in transcript up- and down-regulation during fertilization.
We used microarrays to detail the global program of gene expression in the egg cell at anthesis, complementing prior data on the male germ lineage, and following gene expression during fertilization and gamete fusion.
 
Overall design Female gametophytic cells consisting of isolated unfertilized egg cells (0 hr after pollination = 0 hap = anthesis), in vivo pollinated 1-1.5 hap fertilized egg cells, and in vivo pollinated 2.5-3 hap fertilized zygotes were compared before and soon after plasmogamy (cellular fusion) and karyogamy (nuclear fusion).
keywords: tissue expressional profile, female gametophyte germline, anthesis unfertilized egg cell, fertilized egg cell, fertilized zygote
 
Contributor(s) Russell SD, Gou X
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Feb 17, 2011
Last update date Dec 31, 2022
Contact name Scott D Russell
E-mail(s) srussell@ou.edu
Phone 405 210-8935
Organization name University of Oklahoma
Department Dept of Microbiology and Plant Biology
Street address 770 Van Vleet Oval
City Norman
State/province OK
ZIP/Postal code 73019
Country USA
 
Platforms (1)
GPL2025 [Rice] Affymetrix Rice Genome Array
Samples (9)
GSM677573 Unfertilized Egg Cell, biological rep 1
GSM677574 Unfertilized Egg Cell, biological rep 2
GSM677575 Unfertilized Egg Cell, biological rep 3
Relations
BioProject PRJNA137259

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Supplementary file Size Download File type/resource
GSE27400_RAW.tar 35.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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