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Series GSE277472 Query DataSets for GSE277472
Status Public on Sep 19, 2024
Title Altered bone marrow myelopoiesis contributes to kidney injury [ATAC-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The objectives of this study were to characterize bone marrow alteration in the context of kidney disease.
 
Overall design Mobilized apheresis products were collected from de-identified, healthy donors. Isolated CD34+ HSPCs were cultured in myeloid expansion medium for 10 days with or without TNFα supplement. For bulk ATAC sequencing, CD14+ monocytic cells were further isolated at day 10. The cells were then thawed in a 37oC water bath, pelleted, washed with cold PBS, and tagmented as previously described (Buenrostro et al. 2013), with some modifications based on (Corces et al. 2017).
 
Contributor(s) Hahm E, Spear R
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Sep 18, 2024
Last update date Sep 19, 2024
Contact name Eunsil Hahm
E-mail(s) eunsil_hahm@rush.edu
Organization name Rush University Medical Center
Street address 735 W Harrison St. , 753
City Chicago
ZIP/Postal code 60612
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM8522949 CD14+ cells, untreated, donor 3
GSM8522950 CD14+ cells, untreated, donor 5
GSM8522951 CD14+ cells, untreated, donor 9
Relations
BioProject PRJNA1162493

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Supplementary file Size Download File type/resource
GSE277472_RAW.tar 692.1 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA

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