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Status |
Public on Sep 23, 2024 |
Title |
Contrasting the development of larval and adult body plans during the evolution of biphasic lifecycles in sea urchins |
Organism |
Heliocidaris erythrogramma |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Sea urchins are a unique system for studying developmental transistions because of the stark differences between their bilateral larval and pentaradial adult body plans. Here, we use single cell RNA-sequencing to analyze the development of Heliocidaris erythrogramma (He), a sea urchin species with an accelerated, non-feeding mode of larval development. The sequencing time course extends from early embryogenesis to roughly a day before the onset of metamorphosis in He larvae, which is a period that has not been covered by previous datasets. We find that the non-feeding developmental strategy of He is associated with several changes in the specification of larval cell types compared to sea urchins with feeding larvae, such as the loss of a larva-specific skeletal cell population. Furthermore, the development of the larval and adult body plans in sea urchins may utilize largely different sets of regulatory genes. These findings lay the groundwork for extending existing developmental gene regulatory networks to cover additional stages of biphasic lifecycles.
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Overall design |
5 scRNA-seq samples capturing 5 time points over 14 hours of a lecithotrophic sea urchin development
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Contributor(s) |
McDonald BD, Massri AJ, Berrio A, Byrne M, McClay DR, Wray GA |
Citation(s) |
39465623 |
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Submission date |
Sep 18, 2024 |
Last update date |
Dec 13, 2024 |
Contact name |
Alejandro Berrio |
E-mail(s) |
alebesc@gmail.com
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Phone |
5123489070
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Organization name |
Duke University
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Department |
Biology
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Lab |
Wray
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Street address |
124 Science Dr
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City |
DURHAM |
State/province |
NC |
ZIP/Postal code |
27708 |
Country |
USA |
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Platforms (1) |
GPL34915 |
Illumina NovaSeq 6000 (Heliocidaris erythrogramma) |
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Samples (5)
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Relations |
BioProject |
PRJNA1162557 |
Supplementary file |
Size |
Download |
File type/resource |
GSE277518_RAW.tar |
67.7 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
Raw data are available in SRA |
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