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Series GSE277518 Query DataSets for GSE277518
Status Public on Sep 23, 2024
Title Contrasting the development of larval and adult body plans during the evolution of biphasic lifecycles in sea urchins
Organism Heliocidaris erythrogramma
Experiment type Expression profiling by high throughput sequencing
Summary Sea urchins are a unique system for studying developmental transistions because of the stark differences between their bilateral larval and pentaradial adult body plans. Here, we use single cell RNA-sequencing to analyze the development of Heliocidaris erythrogramma (He), a sea urchin species with an accelerated, non-feeding mode of larval development. The sequencing time course extends from early embryogenesis to roughly a day before the onset of metamorphosis in He larvae, which is a period that has not been covered by previous datasets. We find that the non-feeding developmental strategy of He is associated with several changes in the specification of larval cell types compared to sea urchins with feeding larvae, such as the loss of a larva-specific skeletal cell population. Furthermore, the development of the larval and adult body plans in sea urchins may utilize largely different sets of regulatory genes. These findings lay the groundwork for extending existing developmental gene regulatory networks to cover additional stages of biphasic lifecycles.
 
Overall design 5 scRNA-seq samples capturing 5 time points over 14 hours of a lecithotrophic sea urchin development
 
Contributor(s) McDonald BD, Massri AJ, Berrio A, Byrne M, McClay DR, Wray GA
Citation(s) 39465623
Submission date Sep 18, 2024
Last update date Dec 13, 2024
Contact name Alejandro Berrio
E-mail(s) alebesc@gmail.com
Phone 5123489070
Organization name Duke University
Department Biology
Lab Wray
Street address 124 Science Dr
City DURHAM
State/province NC
ZIP/Postal code 27708
Country USA
 
Platforms (1)
GPL34915 Illumina NovaSeq 6000 (Heliocidaris erythrogramma)
Samples (5)
GSM8523871 Larvae, 36 hours post fertilization
GSM8523872 Larvae, 42 hours post fertilization
GSM8523873 Larvae, 48 hours post fertilization
Relations
BioProject PRJNA1162557

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Supplementary file Size Download File type/resource
GSE277518_RAW.tar 67.7 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA

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