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Series GSE27885 Query DataSets for GSE27885
Status Public on Nov 21, 2011
Title Transcription profile of B cells isolated from peripheral lymph nodes of wild-type vs CCR7-/- mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary We investigated the transcriptional profile of B cells isolated from peripheral lymph nodes of CCR7-/-C57BL/6 and C57BL/6 mice respectively. B cells were sorted as follows: CD19+, CD3-, CD11c-, CD11b-, NK1.1-, GR-1-. Very few genes were differentially regulated in CCR7-/- B cells, however the expression of a number of genes associated with B-cell activation was increased in CCR7-/- B cells compared to WT B cells. Our data suggest a role of CCR7 signaling in B-cell activation processes.
Overall design 12 purified RNA samples (6 samples of each genotype), originated from 12 individually facs-sorted cell samples, were used for the microarray study. 3 RNA samples per genotype were pooled, giving rise to 2 wildtype- and 2 Ccr7 knock-out pools. In each of the 2 dual-color microarray hybridizations, samples from 1 wildtype and 1 Ccr7 knock-out pool were cohybridized. Microarray hybridizations 1 and 2 were performed in a dye-swap approach.
Contributor(s) Moschovakis G, Bubke A, Dittrich-Breiholz O, Kremmer E, Förster R
Citation(s) 21969271
Submission date Mar 10, 2011
Last update date May 10, 2018
Contact name Oliver Dittrich-Breiholz
Organization name Medical School Hannover
Department Research Core Unit Genomics
Street address Carl-Neuberg-Str. 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (2)
GSM688733 B cells WT vs CCR7-/- Replicate 1 (Pool1)
GSM688734 B cells WT vs CCR7-/- Replicate 2 (Pool2)
BioProject PRJNA137997

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Supplementary file Size Download File type/resource
GSE27885_RAW.tar 37.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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