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Series GSE27893 Query DataSets for GSE27893
Status Public on Aug 24, 2011
Title Genome-wide maps of chromatin state in early erythroid precursors versus later, more differentiated erythroblasts.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Here we globally analyzed mRNA and epigenetic changes in both early erythroid progenitors and late erythroblasts. Concomitant with gene induction there was an increase in RNA Pol II binding and activation marks near the transcriptional start site (TSS) and the elongation mark H3K79me2 (but not H3K36me3),both near the TSS and along the full gene length. In contrast, most repressed genes became depleted of elongation marks. We also found that relative changes in histone modification levels--in particular, H3K79me2 and H4K16ac-- most predictive of gene expression patterns. Our data suggests that in terminal erythropoiesis both transcriptional initiation and elongation contribute to induction of erythroid genes; in contrast, gene repression is mainly mediated by inhibition of Pol II elongation. Our data provides a comprehensive map of the epigenome during terminal erythroid differentiation.
 
Overall design Examination of 7 different histone modifications and Pol II binding in 2 cell types.
 
Contributor(s) Wong B, Hattangadi S
Citation(s) 21860024
Submission date Mar 10, 2011
Last update date May 15, 2019
Contact name Bill Wong
E-mail(s) wibr-bioinformatics@wi.mit.edu, pwong@wi.mit.edu
Organization name Whitehead Institute
Lab BaRC
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (18)
GSM688808 H3K4me2pos_ChIPSeq
GSM688809 H3K4me3pos_ChIPSeq
GSM688810 H3K9Acpos_ChIPSeq
This SubSeries is part of SuperSeries:
GSE32111 Gene induction and repression during terminal erythropoiesis are mediated by distinct epigenetic changes.
Relations
BioProject PRJNA153919
SRA SRP036158

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Supplementary file Size Download File type/resource
GSE27893.txt.gz 942 b (ftp)(http) TXT
GSE27893_RAW.tar 409.5 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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