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Series GSE27929 Query DataSets for GSE27929
Status Public on May 24, 2011
Title Functional analysis of Kap1 genomic recruitment
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary A current model for the genomic recruitment of Kap1 is via its interaction with KRAB domain-containing zinc finger transcription factors. We have performed ChIP-seq for various mutant KAP1 proteins and shown that this recruitment mechanism mediates binding of KAP1 only to the 3’ ends of zinc finger genes and that other factors are involved in recruiting KAP1 to promoter regions.
Overall design 17 total ChIP-seq datasets; three different FLAG-KAP1 mutants, one FLAG-KAP1 wild type, and four different Input datasets from 4 different stable cell lines derived from HEK293 cells: 1 FLAG-KAP1 wild type dataset and 1 Input dataset done from HEK293 stable cells; 1 FLAG-KAP1 HP1BDmut dataset and 1 Input dataset done from HEK293 stable cells, 1 FLAG-KAP1 N-ter RBCC{delta}mut dataset and 1 Input dataset done from HEK293 stable cells, 1 FLAG-KAP1 C-ter PB{delta}mut dataset and 1 Input dataset done from HEK293 stable cells. One FLAG-KAP1 N/C-ter (RBCC+PB){delta}mut dataset done from T-REx HEK293 stable cells. One endogenous KAP1 dataset done from HEK293 cells. Two independent ELK4 datasets done from duplicate HEK293 cells. One endogenous Kap1 dataset and one Input dataset from a stable cell line derived from U2OS cells.
Contributor(s) Iyengar S, Farnham PJ
Citation(s) 21343339
Submission date Mar 14, 2011
Last update date May 15, 2019
Contact name Philip Cayting
Organization name Stanford University
Department Genetics
Lab Snyder
Street address 1501 S California Ave
City Palo Alto
State/province CA
ZIP/Postal code 94304
Country USA
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (17)
GSM700353 FLAG-KAP1 wild type_HEK293 stables_ChIP-seq_rep1_lane3,6,7
GSM700354 FLAG-KAP1 HP1BDmut_HEK293 stables_ChIP-seq_rep1_lane4
GSM700355 FLAG-KAP1 N-ter RBCC{delta} mut_HEK293 stables_ChIP-seq_rep1_lane2
SRA SRP006844
BioProject PRJNA137849

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Supplementary file Size Download File type/resource
GSE27929_RAW.tar 8.0 Gb (http)(custom) TAR (of BAM)
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Raw data are available in SRA
Processed data provided as supplementary file

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