|
Status |
Public on May 24, 2011 |
Title |
Functional analysis of Kap1 genomic recruitment |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
A current model for the genomic recruitment of Kap1 is via its interaction with KRAB domain-containing zinc finger transcription factors. We have performed ChIP-seq for various mutant KAP1 proteins and shown that this recruitment mechanism mediates binding of KAP1 only to the 3â ends of zinc finger genes and that other factors are involved in recruiting KAP1 to promoter regions.
|
|
|
Overall design |
17 total ChIP-seq datasets; three different FLAG-KAP1 mutants, one FLAG-KAP1 wild type, and four different Input datasets from 4 different stable cell lines derived from HEK293 cells: 1 FLAG-KAP1 wild type dataset and 1 Input dataset done from HEK293 stable cells; 1 FLAG-KAP1 HP1BDmut dataset and 1 Input dataset done from HEK293 stable cells, 1 FLAG-KAP1 N-ter RBCC{delta}mut dataset and 1 Input dataset done from HEK293 stable cells, 1 FLAG-KAP1 C-ter PB{delta}mut dataset and 1 Input dataset done from HEK293 stable cells. One FLAG-KAP1 N/C-ter (RBCC+PB){delta}mut dataset done from T-REx HEK293 stable cells. One endogenous KAP1 dataset done from HEK293 cells. Two independent ELK4 datasets done from duplicate HEK293 cells. One endogenous Kap1 dataset and one Input dataset from a stable cell line derived from U2OS cells.
|
|
|
Contributor(s) |
Iyengar S, Farnham PJ |
Citation(s) |
21343339 |
|
Submission date |
Mar 14, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Philip Cayting |
E-mail(s) |
pcayting@stanford.edu
|
Organization name |
Stanford University
|
Department |
Genetics
|
Lab |
Snyder
|
Street address |
1501 S California Ave
|
City |
Palo Alto |
State/province |
CA |
ZIP/Postal code |
94304 |
Country |
USA |
|
|
Platforms (1) |
GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
Samples (17)
|
GSM700353 |
FLAG-KAP1 wild type_HEK293 stables_ChIP-seq_rep1_lane3,6,7 |
GSM700354 |
FLAG-KAP1 HP1BDmut_HEK293 stables_ChIP-seq_rep1_lane4 |
GSM700355 |
FLAG-KAP1 N-ter RBCC{delta} mut_HEK293 stables_ChIP-seq_rep1_lane2 |
GSM700356 |
FLAG-KAP1 C-ter PB{delta}_HEK293 stables_ChIP-seq_rep1_lane5 |
GSM700357 |
FLAG-KAP1 N/C-ter (RBCC+PB){delta}_T -Rex HEK293 stables_ChIP-seq_rep1_lane2 |
GSM700358 |
Endogenous KAP1_HEK293 cells_ChIP-seq_rep1_lane3 |
GSM700359 |
Endogenous KAP1_HEK293 cells_ChIP-seq_rep1_lane4 |
GSM700360 |
Endogenous ELK4_HEK293 cells_ChIP-seq_rep1_lane7 |
GSM700361 |
Endogenous ELK4_HEK93 cells_ChIP-seq_rep2_lane8 |
GSM700362 |
Input_1_HEK293 stables_ChIP-seq_rep1_lane6 |
GSM700363 |
Input_2_HEK293 stables_ChIP-seq_rep1_lane6 |
GSM700364 |
Input_3_HEK293 stables_ChIP-seq_rep1_lane5 |
GSM700365 |
Input_4_HEK293 stables_ChIP-seq_rep1_lane6 |
GSM700366 |
FLAG-KAP1 wild type_U2OS stables_ChIP-seq_rep1_lane8 |
GSM700367 |
Input_U2OS stables_ChIP-seq_rep1_lane4 |
GSM700368 |
FLAG-KAP1 wild type_U2OS stables_ChIP-seq_rep2A_lane5 |
GSM700369 |
FLAG-KAP1 wild type_U2OS stables_ChIP-seq_rep2B_lane7 |
|
Relations |
SRA |
SRP006844 |
BioProject |
PRJNA137849 |