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Series GSE27969 Query DataSets for GSE27969
Status Public on Mar 21, 2011
Title ChIP-seq analysis reveals distinct H3K27me3 profiles associated with gene regulation [mRNA profiling]
Organism Mus musculus
Experiment type Expression profiling by array
Third-party reanalysis
Summary Transcriptional control is dependent on a vast network of epigenetic modifications. One epigenetic mark of particular interest is tri-methylation of lysine 27 on histone H3 (H3K27me3), which is catalyzed and maintained by the Polycomb Repressor Complex (PRC2). Although this histone mark is studied widely, the precise relationship between its local pattern of enrichment and regulation of gene expression is currently unclear. We have used ChIP-seq to generate genome wide maps of H3K27me3 enrichment, and have identified three enrichment profiles with distinct regulatory consequences. First, a broad domain of H3K27me3 enrichment across the body of genes corresponds to the canonical view of H3K27me3 as inhibitory to transcription. Second, a peak of enrichment around the transcription start site is commonly associated with “bivalent” genes, where H3K4me3 also marks the TSS. Finally and most surprisingly, we identified an enrichment profile with a peak in the promoter of genes that is associated with active transcription. Genes with each of these three profiles were found in different proportions in each of the cell types studied. The data analysis techniques developed here will be useful for the identification of common enrichment profiles for other histone modifications that have important consequences for transcriptional regulation.
Overall design Expression profiling of Gata1 null Megakaryocyte Erythroid cells (G1ME cells)

Supplementary file: Comprehensive matrix representing the entire gene expression dataset (i.e., GSM691834 and GSM691835, plus the re-analyzed Samples from Series GSE8024).
Contributor(s) Young MD
Citation(s) 21652639
Submission date Mar 15, 2011
Last update date Feb 11, 2019
Contact name Matthew Daniel Young
Organization name Walter & Eliza Hall Institute of Medical Research
Department Bioinformatics
Street address 1G Royal Parade
City Parkville
State/province Victoria
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (2)
GSM691834 G1ME_WT_rep1
GSM691835 G1ME_WT_rep2
This SubSeries is part of SuperSeries:
GSE27970 ChIP-seq analysis reveals distinct H3K27me3 profiles associated with gene regulation
Reanalysis of GSM198062
Reanalysis of GSM198063
Reanalysis of GSM198064
Reanalysis of GSM198070
Reanalysis of GSM198072
Reanalysis of GSM198065
Reanalysis of GSM198066
Reanalysis of GSM198067
BioProject PRJNA141717

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27969_RAW.tar 7.2 Mb (http)(custom) TAR (of CEL)
GSE27969_complete_matrix.txt.gz 2.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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