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Series GSE281404 Query DataSets for GSE281404
Status Public on Jan 24, 2025
Title Deficiency of the palmitoyl acyltransferase ZDHHC7 modulates gene expression in mice after a mild chronic stress paradigm
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Chronic stress (CS) is a debilitating condition that negatively affects body and brain. In mice, CS effects range from changes in behaviour down to the level of gene expression. These effects are partly mediated by sex and sex steroid hormones, which in turn are affected by the palmitoyl acyltransferase ZDHHC7. ZDHHC7 might modulate also the response to CS via palmitoylation of sex steroid hormone receptors and other proteins critical for neuronal structure and functions. Therefore, we aimed to investigate the role of ZDHHC7 in response to CS on different system levels in a mouse model of Zdhhc7-deficiency. Female and male Zdhhc7-knockout (KO) and -wildtype (WT) mice underwent a four-week-mild CS paradigm or non-stress control (C) condition. After C or CS, amongst other analyses, brain gene expression profiles (via mRNA-seq transcriptomics) were investigated. Analyses focused on effects of genotype (KO vs. WT) or condition (C vs. CS) separately in both sexes. Our results revealed significant effects particularly in females. Female KOs showed upregulated cortistatin expression after CS (C vs. CS: p<0.05). Zdhhc7-deficiency seemed to be related to a sex-specific stress response and may reveal genetic factors of CS-resilience in female mice.
 
Overall design Female and male Zdhhc7-KO and -WT mice underwent a four-week-mild CS paradigm or non-stress C condition. After C or CS, mRNA-seq of brain tissues (hippocampus, medial prefrontal cortex) was performed to analyse effects of genotype (KO vs. WT) or condition (C vs. CS) separately in both sexes.

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Submitter states that missing raw files are due to file loss.
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Contributor(s) Hohoff C, Kerkenberg N, Zhang M, Palkowska W, Wachsmuth L, Peng M, Stiehl L, Schettler C, Zang J, Huge A, Ponimaskin E, Faber C, Baune BT, Zhang W
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Submission date Nov 08, 2024
Last update date Jan 24, 2025
Contact name Christa Hohoff
E-mail(s) hohoffch@uni-muenster.de
Organization name University of Muenster
Department Department of Psychiatry
Street address Albert-Schweitzer-Campus 1, A9
City Münster
ZIP/Postal code 48149
Country Germany
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (80)
GSM8619943 Hippocampus, KO, f, CS, 2277, biol rep 1
GSM8619944 Hippocampus, KO, f, CS, 2317, biol rep 2
GSM8619945 Hippocampus, KO, f, CS, 2399, biol rep 3
Relations
BioProject PRJNA1183804

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE281404_RAW.tar 14.7 Mb (http)(custom) TAR (of TXT)
GSE281404_mRNAseq_norm_read_counts.xlsx 33.4 Mb (ftp)(http) XLSX
Raw data not provided for this record

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