|
| Status |
Public on Jun 22, 2012 |
| Title |
Genome-wide RNA expression in muscle-enriched embryos across different mutations. |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the structure of the bodywall muscle differentiation network, we sought to both identify what these transcription factors regulate in bodywall muscle. Through loss of function analysis, we identified genome-wide regulatory targets of hlh-1 and unc-120 using RNA-seq. 1445 targets of hlh-1 and 3674 targets of unc-120 were identified, with 760 of these genes being targets of both. Crosstalk was identified between the networks of hlh-1 and hlh-8, which is involved in non-bodywall muscle development. In the process, we quantified bodywall muscle RNA. Our data suggest that shared target genes and overlapping regulation buffer the hlh-1 and unc-120 mutant phenotypes.
|
| |
|
| Overall design |
RNA expression genome-wide in the early embryo
|
| |
|
| Contributor(s) |
Kuntz S, Wold B |
| Citation(s) |
22730465 |
| |
| Submission date |
Apr 12, 2011 |
| Last update date |
Nov 03, 2017 |
| Contact name |
Diane E Trout |
| Organization name |
California Institute of Technology
|
| Department |
Biology
|
| Lab |
Wold
|
| Street address |
1200 E California Blvd M/C 156-29
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9309 |
Illumina Genome Analyzer (Caenorhabditis elegans) |
|
| Samples (11)
|
|
| This SubSeries is part of SuperSeries: |
| GSE28563 |
HLH-1 binding in muscle-enriched embryos and RNA expression in muscle-enriched embryos across different mutations |
|
| Relations |
| BioProject |
PRJNA143023 |
Less...
More...