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Series GSE28698 Query DataSets for GSE28698
Status Public on Apr 10, 2012
Title Gene expression profiling of imatinib-treated and untreated human Hematopoetic progenitors expressing the ETV6-PDGFRB oncogene
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In order to investigate the mechanism whereby TEL-PDGF-beta (ETV6-PDGFRB) interferes with human hematopoietic progenitors proliferation and differentiation, we analyzed the gene expression response downstream this oncogene. CD34+ cells infected with lentivirus coding for TEL-PDGFRb were cultured for 7 days in the absence of cytokines. Using Affymetrix microarrays, we compared gene expression in these cells and in cells treated for 4 h with low dose imatinib (Glivec), a potent PDGFR inhibitor, to switch off TEL-PDGFRb signaling.
 
Overall design This experiment was performed in three biological replicates. In each replicate gene expression profiling from CD34+ HSCs expressing TEL-PDGFRb untreated with Glivec was compared to the corresoniding treated condition. Total RNA were extracted from transduced CD34+ cells using Trizol reagent (Invitrogen) and the RNeasy kit (QIAGEN).
 
Contributor(s) Montano-Almendras CP, Essaghir A, Demoulin J
Citation(s) 22271894
Submission date Apr 18, 2011
Last update date Mar 25, 2019
Contact name Jean-Baptiste Demoulin
Organization name Université catholique de Louvain
Department de Duve Institute
Lab UCL - MEXP
Street address av Hippocrate 75, B1.74.05
City Brussels
ZIP/Postal code B-1200
Country Belgium
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM710733 Human CD34+ Hematopoetic Stem cells expressing TEL-PDGFRb oncogene – replicate 1
GSM710734 Human CD34+ Hematopoetic Stem cells expressing TEL-PDGFRb oncogene treated with Glivec for 4 hours – replicate 1
GSM710735 Human CD34+ Hematopoetic Stem cells expressing TEL-PDGFRb oncogene – replicate 2
Relations
BioProject PRJNA138911

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28698_RAW.tar 31.9 Mb (http)(custom) TAR (of CEL, CHP, XML)
Processed data included within Sample table
Processed data provided as supplementary file

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