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Series GSE28800 Query DataSets for GSE28800
Status Public on Dec 31, 2011
Title Gene expression data from ABA, [5-(3,4-Dichlorophenyl)Furan-2-yl]-Piperidin-1-ylMethanethione (DFPM), and ABA+DFPM treated Arabidopsis thaliana seedlings.
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Coordinated regulation of protection mechanisms against environmental abiotic stress and pathogen attack is essential for plant adaptation and survival. Initial abiotic stress can interfere with disease resistance signaling. Conversely, initial plant immune signaling may interrupt subsequent ABA signal transduction. However, the processes involved in cross talk between these signaling networks have not been determined. By screening a 9,600 compound chemical library, we identified a small molecule [5-(3,4-Dichlorophenyl)Furan-2-yl]-Piperidin-1-ylMethanethione that rapidly down-regulates ABA-dependent gene expression and also inhibits ABA-induced stomatal closure. Transcriptome analyses show that DFPM also stimulates expression of plant defense-related genes. Plate grown 12-day-old seedlings were transferred into 6 well plates with 1:5000 (V/V) DMSO in water as a control, 30uM DFPM, and 10uM ABA in water as a treatment for 6 hours. DFPM was added 30 min prior to ABA treatment. RNA was extracted using Trizol (Invitrogen, Carlsbad, CA, USA) and further purified using RNeasy Plant RNA purification kit (QIAgen, Valencia, CA, USA). Three biological replicates of ATH1 oligonucleotide arrays were hybridized with labeled samples from 1) wild-type Columbia (WT) untreated, 2) WT with 30uM DFPM treatment, 3) WT with 10uM ABA treatment, 4) WT with 30uM DFPM and 10uM ABA treatment. Each biological replicate was prepared by combining 7 independently-treated samples.
Overall design Seedling treatments, 4 agent, 1 genotype/variation sets.
Contributor(s) Kim T, Schroeder JI
Citation(s) 21620700
Submission date Apr 21, 2011
Last update date Aug 15, 2018
Contact name Julian Schroeder
Organization name UCSD
Department Division of Biological Sciences
Lab Schroeder Laboratory
Street address 9500 Gilman Drive #0116
City La Jolla
State/province CA
ZIP/Postal code 92093-0116
Country USA
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (12)
GSM713249 control_3
GSM713250 control_1
GSM713251 control_2
Affiliated with GSE69995
BioProject PRJNA138821

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28800_RAW.tar 25.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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