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| Status |
Public on Jan 01, 2013 |
| Title |
Streptomyces coelicolor A3(2) strain M600 wild type transcriptome ChIP-chip with the bldC null mutant |
| Platform organism |
Streptomyces coelicolor |
| Sample organism |
Streptomyces coelicolor A3(2) |
| Experiment type |
Genome binding/occupancy profiling by array
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| Summary |
Global regulation by the Streptomyces coelicolor atypical MerR-like transcription factor BldC. BldC is a transcriptional regulator essential for morphological development and antibiotic production in Streptomyces coelicolor. Here we identify the BldC regulon by means of chromatin immunoprecipitation (ChIP) microarray analysis. The BldC regulon encompasses at least 201 transcriptional units, which include many genes that play key roles in Streptomyces development (e.g., bldC itself, bldB, bldM, whiB, whiD, whiI, sigF, smeA-sffA, hupS), antibiotic production (e.g., afsK) and stress response (e.g., clpB, nsrR, sigE, sigF). All BldC-binding sites identified by ChIP-chip are present in the promoters of the target genes. In vitro DNA-binding experiments show that BldC is capable of binding DNA specifically in the absence of other proteins and suggest that BldC is a minor-groove DNA-binding protein. The regulon of BldC partially overlaps with that of the pleiotropic regulator BldD. BldC and BldD bind to distinct sites in the promoter region of smeA, where they simultaneously repress its transcription.
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| Overall design |
ChIP-chip experiment using an anti-BldC antibody and a total DNA control. Comparison of IP in wild-type strain vs. IP in a bldC null mutant strain.
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| Contributor(s) |
den Hengst C, Tran N, Chandra G, Dixon R, Buttner M |
| Citation missing |
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| Submission date |
Apr 28, 2011 |
| Last update date |
Jan 02, 2013 |
| Contact name |
Chris den Hengst |
| E-mail(s) |
chris.den-hengst@bbsrc.ac.uk
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| Phone |
+44 (0) 01603 450796
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| Fax |
+44 (0) 01603 450778
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| URL |
http://intranet/infoserv/cgi-bin/directory/default.asp?k=&q=buttner
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| Organization name |
John Innes Centre
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| Department |
Molecular Microbiology
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| Lab |
Mark Buttner
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| Street address |
Colney
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| City |
Norwich |
| State/province |
Norfolk |
| ZIP/Postal code |
NR4 7UH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL10757 |
Oxford Gene Technology Streptomyces coelicolor 4x44K format |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA140673 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28908_RAW.tar |
26.6 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE28908_final_processed_data.txt.gz |
449.1 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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