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Series GSE28908 Query DataSets for GSE28908
Status Public on Jan 01, 2013
Title Streptomyces coelicolor A3(2) strain M600 wild type transcriptome ChIP-chip with the bldC null mutant
Platform organism Streptomyces coelicolor
Sample organism Streptomyces coelicolor A3(2)
Experiment type Genome binding/occupancy profiling by array
Summary Global regulation by the Streptomyces coelicolor atypical MerR-like transcription factor BldC. BldC is a transcriptional regulator essential for morphological development and antibiotic production in Streptomyces coelicolor. Here we identify the BldC regulon by means of chromatin immunoprecipitation (ChIP) microarray analysis. The BldC regulon encompasses at least 201 transcriptional units, which include many genes that play key roles in Streptomyces development (e.g., bldC itself, bldB, bldM, whiB, whiD, whiI, sigF, smeA-sffA, hupS), antibiotic production (e.g., afsK) and stress response (e.g., clpB, nsrR, sigE, sigF). All BldC-binding sites identified by ChIP-chip are present in the promoters of the target genes. In vitro DNA-binding experiments show that BldC is capable of binding DNA specifically in the absence of other proteins and suggest that BldC is a minor-groove DNA-binding protein. The regulon of BldC partially overlaps with that of the pleiotropic regulator BldD. BldC and BldD bind to distinct sites in the promoter region of smeA, where they simultaneously repress its transcription.
Overall design ChIP-chip experiment using an anti-BldC antibody and a total DNA control. Comparison of IP in wild-type strain vs. IP in a bldC null mutant strain.
Contributor(s) den Hengst C, Tran N, Chandra G, Dixon R, Buttner M
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Submission date Apr 28, 2011
Last update date Jan 02, 2013
Contact name Chris den Hengst
Phone +44 (0) 01603 450796
Fax +44 (0) 01603 450778
URL http://intranet/infoserv/cgi-bin/directory/default.asp?k=&q=buttner
Organization name John Innes Centre
Department Molecular Microbiology
Lab Mark Buttner
Street address Colney
City Norwich
State/province Norfolk
ZIP/Postal code NR4 7UH
Country United Kingdom
Platforms (1)
GPL10757 Oxford Gene Technology Streptomyces coelicolor 4x44K format
Samples (3)
GSM716427 bldc1
GSM716428 bldc2
GSM716429 ctrl
BioProject PRJNA140673

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28908_RAW.tar 26.6 Mb (http)(custom) TAR (of TXT)
GSE28908_final_processed_data.txt.gz 449.1 Kb (ftp)(http) TXT
Processed data included within Sample table

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