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Series GSE28955 Query DataSets for GSE28955
Status Public on Mar 31, 2012
Title A set of 72 differentially expressed miRNAs provide a molecular signature for pancreatic cancer
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary MicroRNAs (miRNAs) are short single-stranded RNA molecules that have a critical role in the regulation of gene expression. Alterations in miRNA expression levels have been observed in multiple tumor types and there is clear evidence on their active involvement in cancer development. Here, a comprehensive miRNA expression profiling in 16 pancreatic cancer cell lines and four normal pancreatic samples provided a specific molecular signature for pancreatic cancer and enabled us to identify 72 differentially expressed miRNAs with approximately half of them being up- and half downregulated in cancer cells as compared to normal samples. Of these, miR-31 was selected for further functional analyses based on its interesting “on-off” type expression profile, i.e. very low or even absent expression in normal pancreas and in six of the pancreatic cancer samples but extremely high expression in the remaining ten cell lines. Quite unexpectedly, both the inhibition of miR-31 in AsPC-1 and HPAF-II pancreatic cancer cells with high endogenous expression and forced expression of miR-31 in MIA PaCa-2 with low endogenous levels led to reduced cell proliferation, migration and invasion. More importantly, in AsPC-1 cells further enhancement of miR-31 also resulted in reduced cell migration and invasion, implicating that the level of miR-31 is critical for these phenotypes. We also identified novel miR-31 target genes, APBB2 and RSBN1, that might contribute to cancer pathogenesis. This study highlights a specific miRNA expression pattern in pancreatic cancer and reveals that manipulation of miR-31 expression leads to reduced cell migration and invasion in pancreatic cancer.
Overall design 16 pancreatic cancer cell lines and 4 normal pancreatic RNA samples were hybridized on Agilent vs1 miRNA microarrays. Pool of four normal samples was hybridized on each slide to allow normalization between slides.
Contributor(s) Laurila EM, Sandstrom S, Rantanen LM, Autio R, Kallioniemi A
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Submission date Apr 29, 2011
Last update date Mar 31, 2012
Contact name Eeva M Laurila
Organization name University of Tampere
Department Institute of Biomedical Technology
Street address Biokatu 6-8
City Tampere
ZIP/Postal code 33580
Country Finland
Platforms (1)
GPL6955 Agilent-016436 Human miRNA Microarray 1.0 (Feature Number version)
Samples (23)
GSM717459 AsPC-1_cancer
GSM717460 BxPC-3_cancer
GSM717461 Capan-1_cancer
BioProject PRJNA140441

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28955_RAW.tar 34.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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