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Series GSE29392 Query DataSets for GSE29392
Status Public on May 19, 2011
Title Gene expression array assay of Lsh WT and Knockout MEF cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary DNA methylation is critical for normal development and plays important roles in genome organization and transcriptional regulation. Although DNA methyltransferases have been identified, the factors that establish and contribute to genome-wide methylation patterns remain elusive. Here, we report a high-resolution cytosine methylation map of the murine genome modulated by Lsh, a chromatin remodeling family member that has previously been shown to regulate CpG methylation at repetitive sequences. We provide evidence that Lsh also controls genome-wide cytosine methylation at nonrepeat sequences and relate those changes to alterations in H4K4me3 modification and gene expression. Deletion of Lsh alters the allocation of cytosine methylation in chromosomal regions of 50 kb to 2 Mb and, in addition, leads to changes in the methylation profile at the 5′ end of genes. Furthermore, we demonstrate that loss of Lsh promotes—as well as prevents—cytosine methylation. Our data indicate that Lsh is an epigenetic modulator that is critical for normal distribution of cytosine methylation throughout the murine genome.
Overall design We used microarrays to detail a correlation between cytosine methylation of murine genome modulated by Lsh at non-repetitive sequence and corresponding gene expression.
Contributor(s) Maunakea A, Che A, Briones V, Lee EY, Geiman T, Huang J, Stephens R, Leighty RM, Zhao K, Muegge K
Citation(s) 21427231
Submission date May 19, 2011
Last update date Jun 21, 2012
Contact name Jigui Shan
Organization name NCI-Frederick
Street address 430 Miller Dr
City Frederick
ZIP/Postal code 21702
Country USA
Platforms (1)
GPL13621 [Mouse430_2_Mm_REFSEQ] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 14]
Samples (2)
GSM726804 Lsh+/+ MEF
GSM726805 Lsh-/- MEF
This SubSeries is part of SuperSeries:
GSE29395 Lsh, chromatin remodeling family member, modulates genome-wide cytosine methylation patterns at nonrepeat sequences
BioProject PRJNA142993

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29392_RAW.tar 5.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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