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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 19, 2011 |
| Title |
Gene expression array assay of Lsh WT and Knockout MEF cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
DNA methylation is critical for normal development and plays important roles in genome organization and transcriptional regulation. Although DNA methyltransferases have been identified, the factors that establish and contribute to genome-wide methylation patterns remain elusive. Here, we report a high-resolution cytosine methylation map of the murine genome modulated by Lsh, a chromatin remodeling family member that has previously been shown to regulate CpG methylation at repetitive sequences. We provide evidence that Lsh also controls genome-wide cytosine methylation at nonrepeat sequences and relate those changes to alterations in H4K4me3 modification and gene expression. Deletion of Lsh alters the allocation of cytosine methylation in chromosomal regions of 50 kb to 2 Mb and, in addition, leads to changes in the methylation profile at the 5′ end of genes. Furthermore, we demonstrate that loss of Lsh promotes—as well as prevents—cytosine methylation. Our data indicate that Lsh is an epigenetic modulator that is critical for normal distribution of cytosine methylation throughout the murine genome.
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| Overall design |
We used microarrays to detail a correlation between cytosine methylation of murine genome modulated by Lsh at non-repetitive sequence and corresponding gene expression.
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| Contributor(s) |
Maunakea A, Che A, Briones V, Lee EY, Geiman T, Huang J, Stephens R, Leighty RM, Zhao K, Muegge K |
| Citation(s) |
21427231 |
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| Submission date |
May 19, 2011 |
| Last update date |
Jun 21, 2012 |
| Contact name |
Jigui Shan |
| E-mail(s) |
shanj@mail.nih.gov
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| Organization name |
NCI-Frederick
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| Street address |
430 Miller Dr
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| City |
Frederick |
| ZIP/Postal code |
21702 |
| Country |
USA |
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| Platforms (1) |
| GPL13621 |
[Mouse430_2_Mm_REFSEQ] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 14] |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE29395 |
Lsh, chromatin remodeling family member, modulates genome-wide cytosine methylation patterns at nonrepeat sequences |
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| Relations |
| BioProject |
PRJNA142993 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29392_RAW.tar |
5.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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