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| Status |
Public on May 26, 2011 |
| Title |
Expression data from Notch-activated CD34+CD45RA-Lin- hematopoietic progenitor cells (HPCs) transduced with nuclear-trapped AF1q/MLLT11 (A2M) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We used microarrays to examine the impact of AF1q/MLLT11 on the gene expression profile of Notch-activated CD34+CD45RA-Lin- HPCs isolated from umbilical cord blood
CD34+CD45RA-Lin- HPCs correspond to early multipotent progenitors. A2M is a nuclear mutant derivative of AF1q/MLLT11
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| |
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| Overall design |
CD34+CD45RA-Lin- HPCs were FACS-sorted, exposed to A2M or control vectors, sorted again based on GFP expression, and cultured for 72 hrs with graded doses of plastic-immobilized Notch ligand Delta1ext-IgG
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| |
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| Contributor(s) |
CANQUE B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 25, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Marc Delord |
| E-mail(s) |
mdelord@gmail.com
|
| Organization name |
Centre hospitalier de Versailles
|
| Department |
Recherche clinique
|
| Street address |
Hopital Saint-Louis, 1 avenue Claude Vellefaux
|
| City |
Paris |
| ZIP/Postal code |
75010 |
| Country |
France |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (6)
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| GSM730681 |
CD34+CD45RA+Lin- HPCs transduced with empty vector cultured onto BSA |
| GSM730682 |
CD34+CD45RA+Lin- HPCs transduced with A2M cultured onto BSA |
| GSM730683 |
CD34+CD45RA+Lin- HPCs transduced with empty vector cultured onto 2 µg of Delta1ext-IgG |
| GSM730684 |
CD34+CD45RA+Lin- HPCs transduced with A2M cultured onto 2 µg of Delta1ext-IgG |
| GSM730685 |
CD34+CD45RA+Lin- HPCs transduced with empty vector cultured onto 5 µg of Delta1ext-IgG |
| GSM730686 |
CD34+CD45RA+Lin- HPCs overexpressing A2M cultured onto 5 µg of Delta1ext-IgG |
|
| Relations |
| BioProject |
PRJNA141477 |
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