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| Status |
Public on Aug 25, 2011 |
| Title |
miRNA expression profiles from E18th rat embryonic striatal stem cells under conditioned environments |
| Platform organism |
synthetic construct |
| Sample organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Background:
Striatum is the crucial unit in brain involved in higher-level organizational learning, motor control and cognitive finctions. It also produces dopaminergic neurons, which play an important role in the control of multiple brain functions including voluntary movement and a span of neurobehavioral processes such as, mood, reward, addiction, and stress. Traumatic brain injury (TBI) lead to striatum related persistent cognitive dysfunctions. A number of cell based therapies such as; induced pluripotent stem cells (ips), neural stem cells (NSCs) and mesenchymal stem cells (MSCs), have been investigated so far for their potential application in TBI. But the journey from bench-to-bedside has not yet been covered sucessfully. As their survival and long-term maintenance in vivo has been huddle so far. So to understand the fate of embryonic striatal stem cells (ESSCs) in long-term neurogenesis in vitro, we have studied the neurogenic potential of rat embryonic striatal stem cells from E18th day embryos under conditioned microenvironments.
Objectives:
To evaluate the neuro-regenerative potential striatum-derived stem cells, we have employed synergestic effects of certain neurotrophins, such as ; EGF, IGF, FGF and LIF as in four combinations (Control/untreated-A, EGF+FGF-B, EGF+FGF+LIF-C, EGF+FGF+IGF-D and EGF+FGF+ IGF +LIF –E) in the microenvironment .
To decipher the regulatory miRNAs involved in ESSC-derived neurogenesis, we have performed detailed miRNA profiling for ESSCs at passage 3 for groupD and group E derived ESSCs (based on their performance in vitro). Out data has explored cruicial mIRNAs involved in strital neurogenesis .
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| Overall design |
Striatum has been shown to control the major part in higher-level organizational aspects of learning in human.
To understand the role of striatum-derived neural progenitors in neural repair, miRNA regulation was trapped for ESSCs under conditioned stimuli.
Among different combinatorial stimuli, group D;(EGF+FGF-2+IGF) and group E; EGF+FGF-2+IGF+LIF) tretaed ESSCs were selected at passage 3 for miRNA analysis experiment.
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| Contributor(s) |
Pati S, Muthuraju S, Abdullah JM, Jaafar H |
| Citation(s) |
25254208 |
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| Submission date |
Jun 28, 2011 |
| Last update date |
Aug 01, 2019 |
| Contact name |
SOUMYA PATI |
| Organization name |
UNIVERSITI SAINS MALAYSIA
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| Street address |
KUBANG KERIAN
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| City |
KOTA BHARU |
| ZIP/Postal code |
16150 |
| Country |
Malaysia |
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| Platforms (1) |
| GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
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| Samples (9)
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| GSM750255 |
E18th rat embryonic striatal stem cells (untreated)_1 |
| GSM750256 |
E18th rat embryonic striatal stem cells (untreated)_2 |
| GSM750257 |
E18th rat embryonic striatal stem cells (untreated)_3 |
| GSM750258 |
E18th rat embryonic striatal stem cells (EGF+FGF+IGF)_1 |
| GSM750259 |
E18th rat embryonic striatal stem cells (EGF+FGF+IGF)_2 |
| GSM750260 |
E18th rat embryonic striatal stem cells (EGF+FGF+IGF)_3 |
| GSM750261 |
E18th rat embryonic striatal stem cells (EGF+FGF+IGF+LIF)_1 |
| GSM750262 |
E18th rat embryonic striatal stem cells (EGF+FGF+IGF+LIF)_2 |
| GSM750263 |
E18th rat embryonic striatal stem cells (EGF+FGF+IGF+LIF)_3 |
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| Relations |
| BioProject |
PRJNA143741 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30276_Processed_summarized_miRNA_data_E18_embryonic_strital_stem_cells.txt.gz |
36.6 Kb |
(ftp)(http) |
TXT |
| GSE30276_RAW.tar |
1.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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