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| Status |
Public on Dec 27, 2012 |
| Title |
The ets transcription factor ELF5 suppresses the estrogen sensitive phenotype and contributes to antiestrogen resistance in luminal breast cancer. [human] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The ets transcription factor ELF5 specifies the differentiation of mammary progenitor cells to establish the milk-secreting lineage. ER- and poor prognosis basal breast cancers arise from this progenitor cell and these cancers express high levels of Elf5. Knockdown of ELF5 expression in basal breast cancer cell lines, or forced expression in luminal breast cancer cell lines, resulted in reduced cell proliferation. Transcript profiling and chromatin immunoprecipitation revealed that the transcriptional activity of ELF5 specified the gene expression patterns that distinguish basal from luminal breast cancer, including suppression of FOXA1, GATA3 and ER, key estrogen-action genes. Tamoxifen treatment of luminal MCF7 cells upregulated Elf5 expression and cells that acquired resistance to Tamoxifen became dependent on ELF5 for proliferation. ELF5 is a regulator of breast cancer cell proliferation, transcriptionally specifies the basal molecular subtype and is utilised by ER+ breast cancer cells to escape proliferative arrest caused by Tamoxifen.
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| Overall design |
Elf5 was knocked down via siRNA in basal HCC1937 cell lines, in triplicate. Elf5 was induced in luminal T47D and MCF7 cell lines via a doxycycline inducible expression vector, in duplicate.
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| Contributor(s) |
Kalyuga M, Gallego-Ortega D, Lee H, Cowley MJ, Caldon L, Stone A, Allerdice S, Valdes-Mora F, Launchbury R, Statham A, Armstrong N, Alles C, Young A, Egger A, Au W, Piggin C, Evans C, Brummer T, Kaplan W, Gee JM, Nicholson RI, Sutherland RL, Swarbrick A, Naylor MJ, Clark S, Carroll J, Ormandy CJ |
| Citation(s) |
23300383 |
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| Submission date |
Jul 05, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Mark Cowley |
| E-mail(s) |
m.cowley@garvan.org.au
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| Organization name |
Garvan Institute of Medical Research
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| Department |
Genome Informatics & Clinical Genomics
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| Lab |
Dinger lab
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| Street address |
384 Victoria St.
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| City |
Darlinghurst |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE30407 |
The ets transcription factor ELF5 suppresses the estrogen sensitive phenotype and contributes to antiestrogen resistance in luminal breast cancer. |
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| Relations |
| BioProject |
PRJNA154827 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30405_RAW.tar |
59.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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