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Series GSE30445 Query DataSets for GSE30445
Status Public on Jul 15, 2011
Title Sox17-transgenic hematopoietic stem cell microarray
Organism Mus musculus
Experiment type Expression profiling by array
Summary The transcription factor SOX17 is expressed by fetal, but not adult hematoipoietic stem cells (HSCs), and is required for the maintenance of fetal and neonatal, but not adult, HSCs. In the current study we show that ectopic expression of Sox17 in adult HSCs and transiently reconstituting multipotent progenitors was sufficient to confer increased self-renewal potential and the expression of fetal HSC genes including fetal HSC surface markers.
To assess the acute effects of ectopic Sox17 expression on global gene expression in adult HSCs, we performed microarray analysis to compare the gene expression profile of adult Sox17-trangenic and control HSCs after short induction of Sox17-transgene expression.
 
Overall design Total RNA were isolated from 5 independent, freshly isolated aliquots of 10,000 HSCs isolated from 8-week old Sox17-transgenic ((tetO)7CMVSox17-IRES-NucEGFP;B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J double transgenic) or littermate control mice that were treated with doxycycline for 5 days to induce transgene expression. Purified RNA was reverse transcribed and amplified using the WT-Ovation™ Pico RNA Amplification system (NuGEN Technologies) following the manufacturer’s instructions. Sense strand cDNA was generated using WT-Ovation™ Exon Module (NuGEN), then fragmented and labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN). 2.5µg of labeled cDNA were hybridized to Affymetrix Mouse Gene ST 1.0 microarrays.
 
Contributor(s) He S, Morrison SJ
Citation(s) 21828271
Submission date Jul 06, 2011
Last update date Mar 04, 2019
Contact name Shenghui He
E-mail(s) hesh@umich.edu
Organization name University of Michigan
Department Life Science institute
Lab Sean Morrison
Street address 210 Washtenaw Avenue, 5258 LSI
City Ann Arbor
State/province Michigan
ZIP/Postal code 48109
Country USA
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (10)
GSM755138 Sox17_transgenic_HSC_Rep1
GSM755139 Sox17_transgenic_HSC_Rep2
GSM755140 Sox17_transgenic_HSC_Rep3
This SubSeries is part of SuperSeries:
GSE30446 Transcription factor SOX17 overexpression in hematopoietic stem cells
Relations
BioProject PRJNA154741

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30445_RAW.tar 40.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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