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Series GSE30501 Query DataSets for GSE30501
Status Public on Dec 05, 2011
Title Down-regulation of PAX2 affects ovarian cancer cell growth and migration
Organism Homo sapiens
Experiment type Expression profiling by array
Summary PAX2 is one of nine PAX genes that regulate tissue development and cellular differentiation in embryos. PAX2 promotes cell proliferation, oncogenic transformation, cell lineage specification, migration, and survival. In our previous study, we found that PAX2 is highly expressed in low-grade ovarian serous carcinoma, but its expression in clear cell, endometrioid, and mucinous cell ovarian carcinomas have not been studied. More importantly, the functional role of PAX2 in ovarian cancer is not known.
Downregulation of PAX2 in PAX2-expressing ovarian cancer cells inhibits cell proliferation and migration. This growth inhibition is due to the upregulation of the tumor suppressor gene G0S2 and subsequent induction of apoptosis. The PAX2 pathway thus represents a potential therapeutic target for PAX2-expressing ovarian carcinomas.
 
Overall design Knockdown PAX2 expression in these cell lines was achieved by lentiviral shRNAs targeting the PAX2 gene. PAX2 stable knockdown cells were characterized for cell proliferation, migration, apoptosis, and gene expression profiles.
 
Contributor(s) Wong K
Citation(s) 23502471
Submission date Jul 08, 2011
Last update date Mar 25, 2019
Contact name Kwong-Kwok Wong
E-mail(s) kkwong@mdanderson.org
Phone 713-792-0229
Organization name UT M. D. Anderson Cancer Center
Department Gynecologic Oncology and Reproductive Medicine
Street address T4-3900, 1515 Holcombe Boulevard
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM756539 parental TOV21G cell line
GSM756540 non-target shRNA puromycin stable TOV21G cell line
GSM756541 PLKO-1 vector control puromycin stable TOV21G cell line
Relations
BioProject PRJNA143339

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30501_RAW.tar 28.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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