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Series GSE30622 Query DataSets for GSE30622
Status Public on Sep 14, 2011
Title Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer [Expression Array]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We report the dual role of FoxA1 in androgen receptor recruitment to the chromatin of androgen responsive prostate cancer cell line LNCaP-1F5 using ChIP-sequencing. Depletion of FoxA1 reprograms both androgen and glucocorticoid receptor recruitment and subsequent gene expression. The ChIP-seq has been performed using AR, FoxA1, GR, H3K4me2 antibodies. We have also mapped the DNaseI-hypersensitive sites (DHS) using deep sequencing.
 
Overall design LNCaP-1F5 cells were depleted of FoxA1 using siRNAs. Parental cells and FoxA1 depleted cells (siFoxA1) were treated with vehicle or 100 nM DHT or (100 nM DEX) for 24 hours followed by RNA isolation and hybridization to Illumina arrays. All the experiments have been performed in biological duplicates. Parental cells treated with DHT (or DEX) were analyzed for differentially expressed genes compared to vehicle treated parental cells. Similarly siFoxA1 cells treated with DHT (or DEX) were analyzed for differentially expressed genes compared to vehicle treated siFoxA1 cells.
 
Contributor(s) Sahu B, Laakso M, Ovaska K, Mirtti T, Lundin J, Rannikko A, Sankila A, Turunen J, Lundin M, Konsti J, Vesterinen T, Nordling S, Kallioniemi O, Hautaniemi S, Jänne OA
Citation(s) 21915096
Submission date Jul 12, 2011
Last update date Aug 16, 2018
Contact name Olli A. Jänne
E-mail(s) olli.janne@helsinki.fi
Phone +358919125040
Organization name University of Helsinki
Department Inst. of Biomedicine/Physiology
Lab Androgen Receptor Laboratory
Street address Haartmaninkatu-8
City Helsinki
ZIP/Postal code FI-00014
Country Finland
 
Platforms (2)
GPL6947 Illumina HumanHT-12 V3.0 expression beadchip
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (16)
GSM759641 Vehicle parental rep1_v3
GSM759642 Vehicle parental rep2_v3
GSM759643 DHT parental rep1_v3
This SubSeries is part of SuperSeries:
GSE30624 Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer
Relations
BioProject PRJNA154783

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30622_RAW.tar 32.4 Mb (http)(custom) TAR
GSE30622_non_normalized_v3.txt.gz 1.7 Mb (ftp)(http) TXT
GSE30622_non_normalized_v4.txt.gz 1.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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