|
| Status |
Public on Nov 06, 2011 |
| Title |
Genome-wide binding map of the HIV Tat protein to the human genome (ChIP-chip) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
The HIV-1 Trans-Activator of Transcription (Tat) protein binds to multiple host cellular factors and greatly enhances the level of transcription of the HIV genome. Here, we report the genome-wide binding map of Tat to the human genome in Jurkat T cells (Jurkat-Tat cells) using chromatin immunoprecipitation combined with next-generation sequencing. cDNA microarray was used to monitor gene expression changes between Jurkat and Jurkat-Tat cells. Additionally, we compared distribution of H3K9ac near gene promoters between Jurkat and Jurkat-Tat cells using ChIP-chip method and hybridized onto Agilent promoter array. Our data reveal that Tat’s interaction with the host genome is more extensive than previously thought, with potentially important implications for the viral life cycle.
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| Overall design |
Expression profiles on Jurkat-Tat cells versus Jurkat cells. ChIP on chip for H3K9ac in Jurkat-Tat versus Jurkat cells. ChIP-seq for HIV-1 Tat protein in Jurkat-Tat cells.
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| Contributor(s) |
Kurdistani S, Marrban C, Su T |
| Citation(s) |
22073215 |
| |
| Submission date |
Jul 18, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Siavash K Kurdistani |
| E-mail(s) |
Skurdistani@mednet.ucla.edu
|
| Phone |
310-794-5194
|
| Fax |
310 206-5272
|
| Organization name |
University of California Los Angeles
|
| Department |
Biological Chemistry
|
| Street address |
615 Charles E. Young Dr. South, 337
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7032 |
Human Promoter ChIP-on-chip Microarray - custom annotation |
|
| Samples (1) |
| GSM762829 |
Jurkat Tat cells vs Jurkat cells H3K9ac ChIP-chip |
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| This SubSeries is part of SuperSeries: |
| GSE30739 |
Genome-wide binding map of the HIV Tat protein to the human genome |
|
| Relations |
| BioProject |
PRJNA154549 |
