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Series GSE30736 Query DataSets for GSE30736
Status Public on Nov 06, 2011
Title Genome-wide binding map of the HIV Tat protein to the human genome (ChIP-chip)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary The HIV-1 Trans-Activator of Transcription (Tat) protein binds to multiple host cellular factors and greatly enhances the level of transcription of the HIV genome. Here, we report the genome-wide binding map of Tat to the human genome in Jurkat T cells (Jurkat-Tat cells) using chromatin immunoprecipitation combined with next-generation sequencing. cDNA microarray was used to monitor gene expression changes between Jurkat and Jurkat-Tat cells. Additionally, we compared distribution of H3K9ac near gene promoters between Jurkat and Jurkat-Tat cells using ChIP-chip method and hybridized onto Agilent promoter array. Our data reveal that Tat’s interaction with the host genome is more extensive than previously thought, with potentially important implications for the viral life cycle.
Overall design Expression profiles on Jurkat-Tat cells versus Jurkat cells. ChIP on chip for H3K9ac in Jurkat-Tat versus Jurkat cells. ChIP-seq for HIV-1 Tat protein in Jurkat-Tat cells.
Contributor(s) Kurdistani S, Marrban C, Su T
Citation(s) 22073215
Submission date Jul 18, 2011
Last update date Mar 23, 2012
Contact name Siavash K Kurdistani
Phone 310-794-5194
Fax 310 206-5272
Organization name University of California Los Angeles
Department Biological Chemistry
Street address 615 Charles E. Young Dr. South, 337
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL7032 Human Promoter ChIP-on-chip Microarray - custom annotation
Samples (1)
GSM762829 Jurkat Tat cells vs Jurkat cells H3K9ac ChIP-chip
This SubSeries is part of SuperSeries:
GSE30739 Genome-wide binding map of the HIV Tat protein to the human genome
BioProject PRJNA154549

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30736_RAW.tar 114.4 Mb (http)(custom) TAR (of TSV, TXT)
Processed data included within Sample table

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