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Series GSE31007 Query DataSets for GSE31007
Status Public on Sep 15, 2011
Title An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming [protein binding microarray]
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Other
Summary Alternative splicing (AS) is a key process underlying the expansion of proteomic diversity and the regulation of gene expression. However, the contribution of AS to the control of embryonic stem cell (ESC) pluripotency is not well understood. Here, we identify an evolutionarily conserved ESC-specific AS event that changes the DNA binding preference of the forkhead family transcription factor FOXP1. We show that the ESC-specific isoform of FOXP1 stimulates the expression of transcription factor genes required for pluripotency including OCT4, NANOG, NR5A2 and GDF3, while concomitantly repressing genes required for ESC differentiation. Remarkably, this isoform also promotes the maintenance of ESC pluripotency and the efficient reprogramming of somatic cells to induced pluripotent stem cells. These results reveal an AS switch that plays a pivotal role in the regulation of pluripotency through the control of critical ESC-specific transcriptional programs.
Overall design Protein binding microarray (PBM) experiments were performed for two isoforms of the DNA binding domain of the human FOXP1 gene. Briefly, the PBMs involved binding GST-tagged DNA-binding proteins to two double-stranded 4*44K Agilent microarrays, each containing a different DeBruijn sequence design, in order to determine their sequence preferences. The method is described in Berger et al., Nature Biotechnology 2006.
Contributor(s) Gabut M, Samavarchi-Tehrani P, Wang X, Slobodeniuc V, O’Hanlon D, Sung H, Alvarez M, Talukder S, Pan Q, Woltjen K, Mazzoni EO, Nedelec S, Wichterle H, Hughes TR, Zandstra P, Nagy A, Wrana JL, Blencowe BJ
Citation(s) 21924763
Submission date Jul 28, 2011
Last update date Jun 10, 2014
Contact name Xinchen Wang
Organization name MIT
Street address 32 Vassar St, D514
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL11260 Agilent custom ME and HK design array [8mer]
Samples (6)
GSM768319 XL1-KH-A_ME
GSM768320 XL1-KH-N_ME
GSM768321 C41-KH-A-1_ME
This SubSeries is part of SuperSeries:
GSE30992 An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming
BioProject PRJNA154621

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31007_RAW.tar 8.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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