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Series GSE31206 Query DataSets for GSE31206
Status Public on Aug 11, 2011
Title Colistin-resistant, lipopolysaccharide-deficient Acinetobacter baumannii responds to lipopolysaccharide loss through increased expression of genes involved in the synthesis and transport of lipoproteins, phospholipids and poly-beta-1,6-N-acetylglucosamine
Organism Acinetobacter baumannii
Experiment type Expression profiling by high throughput sequencing
Summary We recently demonstrated that colistin resistance in Acinetobacter baumannii can result from mutational inactivation of genes essential for lipid A biosynthesis. Consequently, strains harboring these mutations are unable to produce the major Gram negative bacterial surface component, lipopolysaccharide (LPS). To understand how A. baumannii compensates for the lack of LPS, we compared the transcriptional profile of the A. baumannii type strain ATCC19606, to that of an isogenic, LPS-deficient, lpxA mutant strain. Analysis of the expression profiles indicated that the LPS-deficient strain showed increased expression of many genes involved in cell envelope and membrane biogenesis. In particular, up-regulated genes included those involved in the Lol lipoprotein transport system and the Mla-retrograde phospholipid transport system. In addition, genes involved in the synthesis and transport of poly-beta-1,6-N-acetylglucosamine (PNAG) were also up-regulated and a corresponding increase in PNAG production was observed. The LPS-deficient strain also exhibited reduced expression of genes predicted to encode the fimbrial subunit FimA and a type VI secretion system (T6SS). The reduced expression of genes involved in T6SS correlated with the detection of the T6SS-effector protein, AssC, in culture supernatants of the A. baumannii wild-type strain, but not in the LPS-deficient strain. Taken together, these data show that, in response to total LPS loss, A. baumannii alters the expression of critical transport and biosynthesis systems associated with modulating the composition and structure of the bacterial surface.
Overall design Comparison of a gene expression in biological duplicate samples derived from parent bacterial strain to an isogenic mutant strain.
Contributor(s) Henry R, Vithanage N, Harrison P, Seeman T, Coutts S, Moffatt JH, Nation RL, Li J, Harper M, Adler B, Boyce JD
Citation(s) 22024825
Submission date Aug 04, 2011
Last update date May 15, 2019
Contact name Paul Francis Harrison
Organization name Victorian Bioinformatics Consortium
Department Microbiology
Street address Building 76, Monash University, Wellington Rd
City Clayton
State/province VIC
ZIP/Postal code 3800
Country Australia
Platforms (1)
GPL14114 Illumina Genome Analyzer IIx (Acinetobacter baumannii)
Samples (4)
GSM773505 19606R_1
GSM773506 19606R_2
GSM773507 19606S_1
SRA SRP007837
BioProject PRJNA145979

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31206_RAW.tar 3.5 Gb (http)(custom) TAR (of BAI, BAM)
GSE31206_count.txt.gz 141.8 Kb (ftp)(http) TXT
GSE31206_readme.txt.gz 290 b (ftp)(http) TXT
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Processed data are available on Series record
Raw data are available in SRA

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