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Series GSE31220 Query DataSets for GSE31220
Status Public on Sep 05, 2011
Title Polysome-associated mRNA levels upon glucose repletion
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Cell survival in changing environments requires appropriate regulation of gene expression, including post-transcriptional gene regulatory mechanisms. Based on reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P-bodies and can return to active translation. We present evidence contradicting the notion that this model is a widespread and general phenomenon. First, genome-wide measurements of mRNA abundance, translation, and ribosome occupancy following glucose withdrawal show that most mRNAs are lost from the cell coincident with their loss from polysomes. Second, only a very limited sub-population of translationally repressed transcripts, comprising fewer than 400 genes, can be reactivated for translation upon glucose re-addition in the absence of new transcription. This highly selective post-transcriptional regulation could be a mechanism for cells to minimize the energetic costs of reversing gene-regulatory decisions in rapidly changing environments by transiently preserving a pool of transcripts whose translation is rate-limiting for growth.
Overall design Sigma 1278b yeast were grown to OD600=1.0-1.1 in YPD in baffled flasks at 30C with vigorous shaking. Cells were treated with thiolutin to a final concentration of 3ug/mL, harvested by centrifugation, and resuspended in pre-warmed YPA medium lacking glucose. Cells were returned to 30C for shaking for 10 minutes. For the refed samples, glucose was added to a final concentration of 2% and samples were taken after 5 minutes. Polysome preparation and RNA isolation were as described in Experimental Procedues. A common RNA sample, polysomal RNA from cells grown in YPD, was used for one channel of each array.
Contributor(s) Arribere JA, Doudna JA, Gilbert WV
Citation(s) 22152478
Submission date Aug 04, 2011
Last update date Sep 02, 2016
Contact name Joshua A Arribere
Phone 6172533707
Organization name MIT
Department Biology
Lab Gilbert
Street address 31 Ames St
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
Platforms (1)
GPL10045 Agilent Yeast 8X15K conversion (Agilent-016322)
Samples (8)
GSM773986 Transcription shutoff, glucose starved cells, non-polysomal RNA bio rep1
GSM773987 Transcription shutoff, glucose starved cells, polysomal RNA bio rep1
GSM773988 Transcription shutoff, glucose starved, then refed cells, non-polysomal RNA bio rep1
This SubSeries is part of SuperSeries:
GSE31393 Reconsidering Movement of Eukaryotic mRNAs Between Polysomes and P-bodies
BioProject PRJNA154117

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Supplementary file Size Download File type/resource
GSE31220_RAW.tar 31.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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