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Status |
Public on Sep 05, 2011 |
Title |
Polysome-associated mRNA levels upon glucose repletion |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by array Other
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Summary |
Cell survival in changing environments requires appropriate regulation of gene expression, including post-transcriptional gene regulatory mechanisms. Based on reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P-bodies and can return to active translation. We present evidence contradicting the notion that this model is a widespread and general phenomenon. First, genome-wide measurements of mRNA abundance, translation, and ribosome occupancy following glucose withdrawal show that most mRNAs are lost from the cell coincident with their loss from polysomes. Second, only a very limited sub-population of translationally repressed transcripts, comprising fewer than 400 genes, can be reactivated for translation upon glucose re-addition in the absence of new transcription. This highly selective post-transcriptional regulation could be a mechanism for cells to minimize the energetic costs of reversing gene-regulatory decisions in rapidly changing environments by transiently preserving a pool of transcripts whose translation is rate-limiting for growth.
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Overall design |
Sigma 1278b yeast were grown to OD600=1.0-1.1 in YPD in baffled flasks at 30C with vigorous shaking. Cells were treated with thiolutin to a final concentration of 3ug/mL, harvested by centrifugation, and resuspended in pre-warmed YPA medium lacking glucose. Cells were returned to 30C for shaking for 10 minutes. For the refed samples, glucose was added to a final concentration of 2% and samples were taken after 5 minutes. Polysome preparation and RNA isolation were as described in Experimental Procedues. A common RNA sample, polysomal RNA from cells grown in YPD, was used for one channel of each array.
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Contributor(s) |
Arribere JA, Doudna JA, Gilbert WV |
Citation(s) |
22152478 |
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Submission date |
Aug 04, 2011 |
Last update date |
Sep 02, 2016 |
Contact name |
Joshua A Arribere |
E-mail(s) |
arribere@mit.edu
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Phone |
6172533707
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Organization name |
MIT
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Department |
Biology
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Lab |
Gilbert
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Street address |
31 Ames St
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City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02141 |
Country |
USA |
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Platforms (1) |
GPL10045 |
Agilent Yeast 8X15K conversion (Agilent-016322) |
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Samples (8)
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GSM773986 |
Transcription shutoff, glucose starved cells, non-polysomal RNA bio rep1 |
GSM773987 |
Transcription shutoff, glucose starved cells, polysomal RNA bio rep1 |
GSM773988 |
Transcription shutoff, glucose starved, then refed cells, non-polysomal RNA bio rep1 |
GSM773989 |
Transcription shutoff, glucose starved, then refed cells, polysomal RNA bio rep1 |
GSM773990 |
Transcription shutoff, glucose starved cells, non-polysomal RNA bio rep2 |
GSM773991 |
Transcription shutoff, glucose starved cells, polysomal RNA bio rep2 |
GSM773992 |
Transcription shutoff, glucose starved, then refed cells, non-polysomal RNA bio rep2 |
GSM773993 |
Transcription shutoff, glucose starved, then refed cells, polysomal RNA bio rep2 |
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This SubSeries is part of SuperSeries: |
GSE31393 |
Reconsidering Movement of Eukaryotic mRNAs Between Polysomes and P-bodies |
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Relations |
BioProject |
PRJNA154117 |
Supplementary file |
Size |
Download |
File type/resource |
GSE31220_RAW.tar |
31.5 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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