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Series GSE31330 Query DataSets for GSE31330
Status Public on Sep 16, 2011
Title Identification of RNAs associated with the epigenetic activator MLL1 in undifferentiated and differentiated CJ7 mouse embryonic stem cells.
Organism Mus musculus
Experiment type Other
Summary The epigenetic activator “Mixed lineage leukemia 1” (Mll1) is paramount for embryonic development and hematopoiesis. We have used RNA-ChIP-on-chip assays to identify long non-coding RNAs, which associate with MLL1 in differentiating CJ7 mouse embryonic stem cells. We identified the long, non-coding RNA (lncRNA) Mistral (Mira), which originates from the spacer DNA region separating Hoxa6 and Hoxa7. Mira supports transcriptional activation of HOXA6 and HOXA7 by recruiting MLL1 to the HOXA6/HOA7 gene cassette.
 
Overall design (+RA) CJ7 mESCs [+RA, -LIF, - beta-mercaptoethanol [beta-ME]] chiped with polyclonal antibody to MLL1 vs control A: differentiating (-RA) CJ7 mESCs [-LIF, -beta-ME] chiped with antibody to MLL1; control B: undifferentiated (U) CJ7 cells chiped with antibody to MLL1; C: +RA-Control CJ7 cells [+RA, -LIF, -beta-ME] chiped with mouse antiserum; -RA-Control CJ7 cells chiped with rabbit antiserum; U-Control CJ7 cells chiped with rabbit antiserum.

The supplementary bed file contains information showing the association of MLL1 with Mira in differentiating CJ7 mouse embryonic stem cells, and was used as a figure in the manuscript.
 
Contributor(s) Bolotin E, Bertani S
Citation(s) 21925392
Submission date Aug 11, 2011
Last update date Mar 23, 2012
Contact name Frank Sauer
E-mail(s) frank.sauer@ucr.edu
Phone 9518273616
Fax 9518274350
Organization name University of California Riverside
Department Biochemistry
Lab own
Street address 2202A Genomics Building
City Riverside
State/province California
ZIP/Postal code 92521
Country USA
 
Platforms (1)
GPL6449 [Mm35b_P06R] Affymetrix Mouse Tiling 2.0R Set, Array 6
Samples (3)
GSM777088 01_FSBS_MLL+RA
GSM777089 04_FSBS_MLL-RA
GSM777090 05_FSBS_MLLU
Relations
BioProject PRJNA145999

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31330_RAW.tar 141.3 Mb (http)(custom) TAR (of CEL)
GSE31330_esc_plusRAvsMock_undif_RAmin.Mm35b_P06R_v02-1_NCBIv36.NR.bpmap_matscore.bar.gz 31.6 Mb (ftp)(http) BAR
GSE31330_esc_plusRAvsMock_undif_RAmin.bed.gz 180.0 Kb (ftp)(http) BED
Processed data are available on Series record

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