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| Status |
Public on Dec 01, 2012 |
| Title |
Species-specific splicing differences are primarily governed by changes in cis-acting sequences |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We assessed whether human transcripts expressed in an aneuploid mouse that carries human chromosome 21 (HsChr21) are spliced in a human-specific or mouse-specific fashion. In almost all cases, human-specific alternative splicing is maintained in the mouse nucleus. Species-specific splicing therefore appears to be primarily directed by cis-acting elements, rather than changes in the levels or activities of trans-acting factors.
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| Overall design |
Approximately 485 million Illumina 50-nt sequence reads were generated for brain and liver tissues from normal human, Tc0 (wildtype) mouse and Tc1 mouse strains. Sequence reads were mapped to splice junctions and %in levels were estimated.
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| Contributor(s) |
Pan Q, Slobodeniuc V, Barbosa-Morais NL, Wilson MD, Kutter C, Han H, Odom DT, Blencowe BJ |
| Citation(s) |
23258890, 23246434 |
| |
| Submission date |
Aug 17, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Qun Pan |
| E-mail(s) |
qun.pan@utoronto.ca
|
| Organization name |
University of Toronto
|
| Street address |
160 College St.
|
| City |
Toronto |
| ZIP/Postal code |
M5S 3E1 |
| Country |
Canada |
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| Platforms (2) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (6)
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| Relations |
| SRA |
SRP007862 |
| BioProject |
PRJNA145829 |
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