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Series GSE31640 Query DataSets for GSE31640
Status Public on Feb 25, 2021
Title An Esrrb and Nanog Cell Fate Regulatory Module Controlled by Feedback Interactions [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Cell fate decisions during development are governed by multi-factorial regulatory mechanisms including chromatin remodelling, DNA methylation, binding of transcription factors to specific loci, RNA transcription and protein synthesis. However, the mechanisms by which such regulatory 'dimensions' coordinate cell fate decisions are currently poorly understood. Here we quantified the multi-dimensional molecular changes that occur in mouse embryonic stem cells (mESCs) upon depletion of Estrogen related receptor beta (Esrrb), a key pluripotency regulator. Analyses of coordinated expression changes identified strikingly different epigenetic regulatory patterns of pluripotency and differentiation states. Comparative analyses of expression changes subsequent to depletion of Esrrb or Nanog, indicated that a system of interlocked feed-forward loops involving both factors plays a central part in regulating the timing of mESC fate decisions. Taken together, our meta-analyses support a hierarchical model in which pluripotency is maintained by an Oct4-Sox2 regulatory module, while the timing of differentiation is regulated by a Nanog-Esrrb
Overall design Genome-wide study of Esrrb binding sites. Examination of 2 different histone modifications in a time series after Esrrb knockdown.
Contributor(s) Sevilla A, Papatsenko D, Mazloom A, Xu H, Vasileva A, Unwin R, LeRoy G, Chen E, Garrett F, Lee D, Trinite B, Webb RL, Wang Z, Su J, Gingold J, Melnick A, Garcia B, Whetton A, MacArthur BD, Ma’ayan A, Lemischka IR
Citation(s) 33816475
Submission date Aug 24, 2011
Last update date Nov 23, 2021
Contact name Ana Sevilla
Phone +34 675348398
Organization name Universidad de Barcelona
Department Departamento de Biologia Celular, Fisiologia e Inmunologia
Lab 4 Floor.
Street address Avda Diagonal 643. Edificio Malagef 4rd Floor, Faculty of Biology
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (14)
GSM785839 1_Esrrb ChIP-Seq_mES_sequence
GSM785840 2_Input DNA_mES_sequence
GSM785841 3_H3K4me3_ChIP_Seq_Day0_mES_s_8_sequence
This SubSeries is part of SuperSeries:
GSE31842 An Esrrb and Nanog Cell Fate Regulatory Module Controlled by Feed Forward Loop Interactions.
SRA SRP007944
BioProject PRJNA155017

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Supplementary file Size Download File type/resource
GSE31640_RAW.tar 662.9 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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