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Series GSE31820 Query DataSets for GSE31820
Status Public on Sep 02, 2011
Title Targets of Ty3 transposition in Saccharomyces cerevisiae
Organism Saccharomyces cerevisiae
Experiment type Genome variation profiling by high throughput sequencing
Summary The goal of these experiments was to define the targets of Ty3 transposition in Saccharomyces cerevisiae. Ty3 is a retroviruslike element that is found at the transcription initiation site of chromosomal tRNA genes.
Overall design A Ty3 that can be induced by growth in galactose-containing medium and which was marked by an insertion of HIS3 downstream of the second open reading frame of the element (POL3) was induced to undergo transposition by plating cells onto galactose containing medium and replica-plating onto medium selective for cells that had undergone transposition. These cells were collected, DNA was extracted, and inverse PCR was performed using primers inside the Ty3 element in order to generate a library of insertion sites flanked by Illumina sequence-compatible primers.
Contributor(s) Qi X, Daily K, Nguyen K, Wang H, Forouzan S, Rigor P, Johnston M, Mitra R, Baldi P, Sandmeyer S
Citation(s) 22287102
Submission date Sep 01, 2011
Last update date May 15, 2019
Contact name Suzanne Sandmeyer
Organization name University of CA, Irvine
Department Biological Chemistry
Lab Sandmeyer
Street address University
City Irvine
State/province CA
ZIP/Postal code 92697-1700
Country USA
Platforms (1)
GPL13272 Illumina Genome Analyzer IIx (Saccharomyces cerevisiae)
Samples (2)
GSM789268 yMA1322_pKN3050
GSM789269 yMA1356_pKN3050
SRA SRP008117
BioProject PRJNA145135

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Supplementary file Size Download File type/resource
GSE31820_RAW.tar 147.9 Mb (http)(custom) TAR (of TXT, WIG)
GSE31820_Supplemental_Table_S3A_AllClusters_0_singles_final.txt.gz 36.8 Kb (ftp)(http) TXT
GSE31820_Supplemental_Table_S3A_and_3B_legend_only.pdf.gz 8.2 Kb (ftp)(http) PDF
GSE31820_Supplemental_Table_S3B_AllClusters_0_multiples_final.txt.gz 8.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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