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Series GSE32673 Query DataSets for GSE32673
Status Public on Jan 04, 2012
Title E2F7 represses a transcriptional network of oscillating cell cycle genes to control S-phase progression.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary E2F transcription factors are known to be important for timely activation of G1/S and G2/M genes required for cell cycle progression, but transcriptional mechanisms for deactivation of cell cycle regulated genes are unknown. Here, we show that E2F7 is highly expressed during mid to late S-phase, occupies promoters of G1/S regulated genes and represses its transcription. ChIP-seq analysis revealed that E2F7 binds preferentially to genomic sites containing the TTCCCGCC motif, which closely resemble the E2F consensus site. We identified 89 target genes that carry E2F7 binding sites close to the transcriptional start site and that are directly repressed by short term induction of E2F7. Most of these target genes are known to be activated by E2Fs and are involved in DNA replication, metabolism and DNA repair. Importantly, induction of E2F7 during G0-G1/S resulted in S-phase arrest and DNA damage, whereas expression of E2F7 during G2/M failed to disturb cell cycle progression. These findings provide strong evidence that E2F7 controls directly the downswing of oscillating G1/S genes during S-phase progression.
 
Overall design 2 samples: E2F7 ChIP-seq and input control, E2F7 ChIP-seq sample was sequenced in 2 independent runs and data were combined for the analysis. Additional sample on Dec. 29, 2015: E2F8 ChIP-seq sample.
 
Contributor(s) Mokry M, Westendorp B, Koerkamp MG, Holstege F, Cuppen E, de Bruin A
Citation(s) 22180533, 27454291
Submission date Oct 06, 2011
Last update date May 15, 2019
Contact name Michal Mokry
E-mail(s) m.mokry@umcutrecht.nl
Organization name Wilhelmina Children's Hospital, University Medical Center Utrecht
Street address Lundlaan 6
City Utrecht
ZIP/Postal code 3584 EA
Country Netherlands
 
Platforms (2)
GPL9442 AB SOLiD System 3.0 (Homo sapiens)
GPL20148 AB 5500xl-W Genetic Analysis System (Homo sapiens)
Samples (3)
GSM810993 E2F7 in HeLa cells run1 and run2
GSM810994 Input HeLa cells
GSM1986068 E2F8 in HeLa cells
Relations
SRA SRP008737
BioProject PRJNA147031

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Supplementary file Size Download File type/resource
GSE32673_RAW.tar 6.4 Gb (http)(custom) TAR (of BAM, COD)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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