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Series GSE3284 Query DataSets for GSE3284
Status Public on Sep 14, 2005
Title A network-based analysis of systemic inflammation in humans
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Oligonucleotide and complementary DNA microarrays are being used to subclassify histologically similar tumours, monitor disease progress, and individualize treatment regimens. However, extracting new biological insight from high-throughput genomic studies of human diseases is a challenge, limited by difficulties in recognizing and evaluating relevant biological processes from huge quantities of experimental data. Here we present a structured network knowledge-base approach to analyse genome-wide transcriptional responses in the context of known functional interrelationships among proteins, small molecules and phenotypes. This approach was used to analyse changes in blood leukocyte gene expression patterns in human subjects receiving an inflammatory stimulus (bacterial endotoxin). We explore the known genome-wide interaction network to identify significant functional modules perturbed in response to this stimulus. Our analysis reveals that the human blood leukocyte response to acute systemic inflammation includes the transient dysregulation of leukocyte bioenergetics and modulation of translational machinery. These findings provide insight into the regulation of global leukocyte activities as they relate to innate immune system tolerance and increased susceptibility to infection in humans.
Keywords: Gene expression profiling of human blood leukocytes in response to in vivo endotoxin administration.
Overall design Healthy male and female subjects were intravenously administered either endotoxin or only vehicle (control). Arterial blood samples were collected before infusion (0 hours) and at post infusion times of 2, 4, 6, 9, and 24 hours. Blood leukocytes were isolated and analyzed using Affymetrix GeneChip arrays.
Web link
Contributor(s) Calvano SE, Xiao W, Richards DR, Felciano RM, Baker HV, Cho RJ, Chen RO, Brownstein BH, Cobb JP, Tschoeke SK, Miller-Graziano C, Moldawer LL, Mindrinos MN, Davis RW, Tompkins RG, Lowery SF
Citation(s) 16136080, 20233835, 19274080, 23383223
Submission date Sep 09, 2005
Last update date Feb 12, 2020
Contact name Wenzhong Xiao, Inflammation & the Host Response to Injury
Phone 617 726 0082
Organization name Massachusetts General Hospital
Department Department of Surgery
Lab Burn & Trauma Research
Street address 55 Fruit Street, GRV 1302
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (3)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL97 [HG-U133B] Affymetrix Human Genome U133B Array
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (110)
GSM73423 CR1_Lysis_0_1626809_11996035_A_
GSM73424 CR1_Lysis_0_21572677_22931099_A_
GSM73425 CR1_Lysis_0_24581684_33693110_A_
BioProject PRJNA92945

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3284_RAW.tar 465.7 Mb (http)(custom) TAR (of CEL, EXP)
GSE3284_sample_information.txt.gz 623 b (ftp)(http) TXT

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