 |
 |
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Oct 01, 2013 |
Title |
Response of Sweet Orange (Citrus sinensis) to Candidatus Liberibacter asiaticus Infection |
Organism |
Citrus sinensis |
Experiment type |
Expression profiling by array
|
Summary |
Citrus greening or huanglongbing (HLB) is a devastating disease of citrus. HLB is associated with the phloem-limited fastidious prokaryotic alpha-proteobacterium Candidatus Liberibacter spp. In this report, we used sweet orange (Citrus sinensis) leaf tissue infected with 'Ca. Liberibacter asiaticus' and compared this with healthy controls. Investigation of the host response was examined with citrus microarray hybridization based on 30,171 sets expressed sequence tag sequences from several citrus species and hybrids. The microarray analysis indicated that HLB infection significantly affected expression of 624 genes whose encoded proteins were categorized according to function. The categories included genes associated with sugar metabolism, plant defense, phytohormone, and cell wall metabolism, as well as 14 other gene categories.
|
|
|
Overall design |
Young, healthy Valencia sweet orange (C. sinensis) plants were graft inoculated with budwood from Ca. L. asiaticus-infected citrus plants. Prior to the innocualtion, the plants were confirmed to be Ca. L. asiaticus-free in ordinary and quantitative PCR tests. The presence of the bacteria in the inoculated plants was confirmed in both conventional and quantitative PCR with specific primers to Ca. L. asiaticus. The stem and root samples used for RNA extraction and hybridization on Affymetrix microarrays were obtained from three symptomatic and three healthy control trees of similar size, approximately 1 year after inoculation.
|
|
|
Contributor(s) |
Aritua V, Wang N |
Citation(s) |
19055434 |
|
Submission date |
Oct 14, 2011 |
Last update date |
Sep 09, 2014 |
Contact name |
Valente Aritua |
E-mail(s) |
varitua@ufl.edu
|
Phone |
863-956-1151
|
Organization name |
University of Florida
|
Department |
Plant Pathology
|
Street address |
700 Experiment Staion Road
|
City |
Lake Alfred |
State/province |
Florida |
ZIP/Postal code |
33850 |
Country |
USA |
|
|
Platforms (1) |
GPL5731 |
[Citrus] Affymetrix Citrus Genome Array |
|
Samples (6)
|
GSM818240 |
Leaves from healthy plant 1, biological rep1 |
GSM818241 |
Leaves from healthy plant 2, biological rep2 |
GSM818242 |
Leaves from healthy plant 3, biological rep3 |
GSM818243 |
Leaves from Ca. L. asiaticus infected plant 4, biological rep1 |
GSM818244 |
Leaves from Ca. L. asiaticus infected plant 5, biological rep2 |
GSM818245 |
Leaves from Ca. L. asiaticus infected plant 6, biological rep3 |
|
Relations |
BioProject |
PRJNA148001 |
Supplementary file |
Size |
Download |
File type/resource |
GSE33003_RAW.tar |
23.6 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
|
|
|
|
 |