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| Status |
Public on Oct 18, 2011 |
| Title |
Expression profile of third instar larval salivary gland tissue |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We use mRNA-seq to transcriptionally profile larval salivary gland tissue from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts.
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| Overall design |
Salivary glands were dissected from 200 wandering third instar larvae and the associated fat body was removed.Salivary glands were transferred to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 10 ug of total RNA and subject to Illumina based sequencing.
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| Contributor(s) |
Sher N, Li S, Bell G, Nordman J, Eng T, Eaton M, MacAlpine D, Orr-Weaver TL |
| Citation(s) |
22090375 |
| |
| Submission date |
Oct 17, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Terry L. Orr-Weaver |
| E-mail(s) |
weaver@wi.mit.edu
|
| Phone |
617-258-5251
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Orr-Weaver
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9061 |
Illumina Genome Analyzer II (Drosophila melanogaster) |
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| Samples (4)
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| GSM818470 |
SuUR-/- larval salivary gland rep 2 |
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| This SubSeries is part of SuperSeries: |
| GSE31900 |
Developmental Control of Gene Copy Number by Repression of Replication Initiation and Fork Progression |
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| Relations |
| SRA |
SRP008977 |
| BioProject |
PRJNA149515 |
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