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| Status |
Public on Jan 01, 2013 |
| Title |
ABI3 TARGET approach |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Deciphering gene regulatory networks (GRNs) is a key for understanding gene expression regulations in living systems. Here, we describe the investigation of the ABSCISIC ACID INSENSITIVE 3 (ABI3) plant transcription factor GRN vicinity by a technique called Network Walking. The method involves transient transformation of protoplasts and inducible nuclear re-localization of transcription factors along with transcriptomic analysis. This genome-wide approach allowed the de novo recovery of i) direct and indirect ABI3 target genes, ii) cis-binding site preference, and iii) biological processes regulated by this canonical abscisic acid response factor. This work improves our knowledge of ABI3 action by inferring network motifs (such as Feed Forwar Loops) under its influence. The novel high-throughput-oriented technique will help accelerate GRN systems investigations in plants, as well as in other organisms.
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| Overall design |
This work studies ABI3 direct and indirect targets by a technique named Network Walking. Root/protoplasts were treated with or without dexamethasone (DEX) and cycloheximide (CHX). 3 reps each.
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| Contributor(s) |
Bargmann B, Coruzzi GM, Krouk G |
| Citation(s) |
23335732 |
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| Submission date |
Oct 31, 2011 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Gabriel Krouk |
| Organization name |
CNRS
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| Department |
Biology
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| Lab |
B&PMP
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| Street address |
2 Place Pierre Viala
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| City |
Montpellier |
| ZIP/Postal code |
34060 |
| Country |
France |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (12)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA148975 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33344_RAW.tar |
21.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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