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Series GSE33344 Query DataSets for GSE33344
Status Public on Jan 01, 2013
Title ABI3 TARGET approach
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Deciphering gene regulatory networks (GRNs) is a key for understanding gene expression regulations in living systems. Here, we describe the investigation of the ABSCISIC ACID INSENSITIVE 3 (ABI3) plant transcription factor GRN vicinity by a technique called Network Walking. The method involves transient transformation of protoplasts and inducible nuclear re-localization of transcription factors along with transcriptomic analysis. This genome-wide approach allowed the de novo recovery of i) direct and indirect ABI3 target genes, ii) cis-binding site preference, and iii) biological processes regulated by this canonical abscisic acid response factor. This work improves our knowledge of ABI3 action by inferring network motifs (such as Feed Forwar Loops) under its influence. The novel high-throughput-oriented technique will help accelerate GRN systems investigations in plants, as well as in other organisms.
Overall design This work studies ABI3 direct and indirect targets by a technique named Network Walking. Root/protoplasts were treated with or without dexamethasone (DEX) and cycloheximide (CHX). 3 reps each.
Contributor(s) Bargmann B, Coruzzi GM, Krouk G
Citation(s) 23335732
Submission date Oct 31, 2011
Last update date Aug 15, 2018
Contact name Gabriel Krouk
Organization name CNRS
Department Biology
Street address 2 Place Pierre Viala
City Montpellier
ZIP/Postal code 34060
Country France
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (12)
GSM824796 -DEX -CHX, rep1
GSM824797 -DEX -CHX, rep2
GSM824798 -DEX -CHX, rep3
Affiliated with GSE69995
BioProject PRJNA148975

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33344_RAW.tar 21.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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