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Series GSE33401 Query DataSets for GSE33401
Status Public on Nov 22, 2011
Title Transcriptome analysis of wild barley (H. vulgare ssp spontaneum) after pathogen inoculation
Organism Hordeum vulgare
Experiment type Expression profiling by array
Summary Hordeum vulgare ssp. spontaneum, accession Shechem 12-32, was submitted to 4 experimental treatments (C. sativus (spot blotch), P. hordei (leaf rust), and water and oil controls) to examine gene transcription differences triggered by biotrophic and hemi-biotrophic pathogens. Inoculated plants were arranged in a split plot design. Samples were collected at 12, 24, 36, & 48 hours after inoculation. A total of 48 samples (4 treatments x 4 time points x 3 replicates) were subjected to GeneChip analysis. Made public: 2009-12-02 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ben Millett. The equivalent experiment is BB61 at PLEXdb.]
 
Overall design treated or untreated: C. sativus (spot blotch) - time: 12 hrs(3-replications); treated or untreated: C. sativus (spot blotch) - time: 24 hrs(3-replications); treated or untreated: C. sativus (spot blotch) - time: 36 hrs(3-replications); treated or untreated: C. sativus (spot blotch) - time: 48 hrs(3-replications); treated or untreated: water control - time: 12 hrs(3-replications); treated or untreated: water control - time: 24 hrs(3-replications); treated or untreated: water control - time: 36 hrs(3-replications); treated or untreated: water control - time: 48 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 12 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 24 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 36 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 48 hrs(3-replications); treated or untreated: oil control - time: 12 hrs(3-replications); treated or untreated: oil control - time: 24 hrs(3-replications); treated or untreated: oil control - time: 36 hrs(3-replications); treated or untreated: oil control - time: 48 hrs(3-replications)
Web link http://www.plexdb.org/modules/PD_browse/experiment_browser.php?experiment=BB61
 
Contributor(s) Millett B, Muehlbauer G
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 02, 2011
Last update date Mar 23, 2012
Contact name PLEXdb Curator, Sudhansu Dash
Organization name Iowa State University
Department Virtual Reality Application Center
Lab PLEXdb
Street address 1023 Crop Genomes Informatics Lab (Iowa State University)
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL1340 [Barley1] Affymetrix Barley Genome Array
Samples (48)
GSM826211 treated or untreated: C. sativus (spot blotch) - time: 12 hrs - rep1
GSM826212 treated or untreated: C. sativus (spot blotch) - time: 12 hrs - rep2
GSM826213 treated or untreated: C. sativus (spot blotch) - time: 12 hrs - rep3
Relations
BioProject PRJNA149143

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33401_RAW.tar 158.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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