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Series GSE33495 Query DataSets for GSE33495
Status Public on Jun 18, 2012
Title Disrupted transcripitonal network in ΔNp63 AEC tissue model [gene expression]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The transcriptional basis for disrupted epidermal differentiation arising from TP63 AEC mutations remains to be elucidated. Here we present an organotypic model of AEC dysfunction that phenocopies differentiation defects observed in AEC patient skin. Transcriptional analysis of model AEC tissue revealed impaired induction of differentiation regulators, including OVOL1, GRHL3, KLF4, PRDM1 and ZNF750. Genome wide binding analyses of TP63 during epidermal differentiation showed direct binding of OVOL1, GRHL3, and ZNF750 promoters suggesting AEC mutants prevent normal activation of these targets by direct transcriptional interference. Remarkably, exogenous ZNF750 restores impaired epidermal differentiation caused by AEC mutation. Thus, repression of ZNF750 is central to disrupted epidermal differentiation in model AEC tissue.
 
Overall design Gene expression analysis: To establish a differentiation signature for primary human keratinocytes, with p63i-depleted, and ΔNp63α AEC mutants overexpressed, total RNA was isolated in biologic duplicate from cells in different conditions and hybridized to Affymetrix HG-U133 2.0 Plus arrays.
 
Contributor(s) Zarnegar BJ, Webster DE, Hunt BV, Yan KJ, Qu K, Berk D, Sen GL, Khavari PA
Citation(s) 22922031
Submission date Nov 04, 2011
Last update date Oct 11, 2022
Contact name Douglas Porter
Organization name Stanford
Department Dermatology
Lab Khavari
Street address 269 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (10)
GSM828656 Keratinocytes_siRNA control_rep1
GSM828657 Keratinocytes_siRNA control_rep2
GSM828658 Keratinocytes_p63i_rep1
This SubSeries is part of SuperSeries:
GSE33572 Disrupted transcripitonal network in ΔNp63 AEC tissue model
Relations
BioProject PRJNA154281

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33495_RAW.tar 46.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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