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Series GSE33571 Query DataSets for GSE33571
Status Public on Jun 18, 2012
Title Disrupted transcriptional network in ΔNp63 AEC tissue model [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The transcriptional basis for disrupted epidermal differentiation arising from TP63 AEC mutations remains to be elucidated. Here we present an organotypic model of AEC dysfunction that phenocopies differentiation defects observed in AEC patient skin. Transcriptional analysis of model AEC tissue revealed impaired induction of differentiation regulators, including OVOL1, GRHL3, KLF4, PRDM1 and ZNF750. Genome wide binding analyses of TP63 during epidermal differentiation showed direct binding of OVOL1, GRHL3, and ZNF750 promoters suggesting AEC mutants prevent normal activation of these targets by direct transcriptional interference. Remarkably, exogenous ZNF750 restores impaired epidermal differentiation caused by AEC mutation. Thus, repression of ZNF750 is central to disrupted epidermal differentiation in model AEC tissue.
Overall design ChIP-Seq analysis: Examination of p63 binding in proliferating and differentiating human keratinocytes
Contributor(s) Zarnegar BJ, Webster DE, Hunt BV, Qu K, Yan KJ, Berk DR, Sen GL, Khavari PA
Citation(s) 22922031
Submission date Nov 09, 2011
Last update date Oct 11, 2022
Contact name Douglas Porter
Organization name Stanford
Department Dermatology
Lab Khavari
Street address 269 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (3)
GSM830120 Input
GSM830121 p63 Progenitor
GSM830122 p63 Differentiated
This SubSeries is part of SuperSeries:
GSE33572 Disrupted transcripitonal network in ΔNp63 AEC tissue model
SRA SRP009255
BioProject PRJNA154341

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Supplementary file Size Download File type/resource
GSE33571_RAW.tar 2.7 Gb (http)(custom) TAR (of BED, BW, SAM)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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