|
| Status |
Public on Jun 04, 2013 |
| Title |
Distinct effects of topoisomerase II inhibitors on tumor cell lines (part 1) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells. Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ´open´ or loosely packed chromosomal areas reflecting exon and promoter regions. As a result, the epigenome and the transcriptome are strongly affected.
|
| |
|
| Overall design |
Tissue culture cells were treated with doxorubicin, aclarubicin or etoposide for 2 hours. Then drugs were removed by extensive washing. cells were further cultured for indicated days before total RNA were extracted and compared to un-treated control.
|
| |
|
| Contributor(s) |
Pang B, Velds A, Kerkhoven R, Neefjes J |
| Citation(s) |
23715267 |
| |
| Submission date |
Nov 11, 2011 |
| Last update date |
Feb 18, 2014 |
| Contact name |
Baoxu Pang |
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Lab |
Michael Snyder
|
| Street address |
3165 Porter Drive
|
| City |
Palo Alto |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13376 |
Illumina HumanWG-6 v2.0 expression beadchip |
|
| Samples (14)
|
| GSM831599 |
MelJuSo cells un-treated |
| GSM831600 |
MelJuSo cells 1 day post doxorubicin rep 1 |
| GSM831601 |
MelJuSo cells 1 day post doxorubicin rep 2 |
| GSM831602 |
MelJuSo cells 1 day post etoposide rep 1 |
| GSM831604 |
MelJuSo cells 1 day post etoposide rep 2 |
| GSM831605 |
MelJuSo cells 2 days post doxorubicin |
| GSM831606 |
MelJuSo cells 2 days post etoposide |
| GSM831607 |
MelJuSo cells 6 days post doxorubicin |
| GSM831608 |
MelJuSo cells 6 days post etoposide |
| GSM831610 |
SW620 cells un-treated |
| GSM831611 |
SW620 cells 1 day post doxorubicin |
| GSM831612 |
SW620 cells 1 day post etoposide |
| GSM831613 |
SW620 cells 6 days post doxorubicin |
| GSM831614 |
SW620 cells 6 days post etoposide |
|
| This SubSeries is part of SuperSeries: |
| GSE33634 |
Topoisomerase II inhibitors and histone eviction |
|
| Relations |
| BioProject |
PRJNA154321 |
Less...
More...