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Status |
Public on Jun 04, 2013 |
Title |
Distinct effects of topoisomerase II inhibitors on tumor cell lines (part 2) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells. Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ´open´ or loosely packed chromosomal areas reflecting exon and promoter regions. As a result, the epigenome and the transcriptome are strongly affected.
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Overall design |
Tissue culture cells were treated with doxorubicin, aclarubicin or etoposide for 2 hours. Then drugs were removed by extensive washing. cells were further cultured for indicated days before total RNA were extracted and compared to un-treated control.
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Contributor(s) |
Pang B, Velds A, Kerkhoven R, Neefjes J |
Citation(s) |
23715267 |
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Submission date |
Nov 11, 2011 |
Last update date |
Feb 18, 2019 |
Contact name |
Baoxu Pang |
Organization name |
Stanford University
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Department |
Genetics
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Lab |
Michael Snyder
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Street address |
3165 Porter Drive
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City |
Palo Alto |
State/province |
CALIFORNIA |
ZIP/Postal code |
94304 |
Country |
USA |
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Platforms (1) |
GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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Samples (4)
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GSM831673 |
MelJuSo cells 1 day post etoposide + aclarubicin |
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This SubSeries is part of SuperSeries: |
GSE33634 |
Topoisomerase II inhibitors and histone eviction |
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Relations |
BioProject |
PRJNA154323 |