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Series GSE33661 Query DataSets for GSE33661
Status Public on Jan 15, 2012
Title Expression analysis of Ralstonia solanacearum strain UW551 at 20°C and 28°C in rich medium (CPG) and in planta
Organism Ralstonia solanacearum UW551
Experiment type Expression profiling by array
Expression profiling by genome tiling array
Summary Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain UW551 at 20°C and 28°C in culture and in planta. The temperatel strain UW551 can wilt and cause full-blown disease on tomato plants at 28°C as well as at 20°C.
 
Overall design A 16 array study using total RNA recovered from the following: 8 separate cultures of Ralstonia solanacearum strain UW551 grown in rich medium-CPG (4 grown at 20°C and 4 grown at 28°C) 8 separate in planta samples of Ralstonia solanacearum strain UW551 harvested from diseased tomato plants cv. BonnyBest (4 recovered from plants grown at 20°C and 4 from plants grown at 28°C) Each array (4-plex format) measures the expression level of 4318 genes from Ralstonia solanacearum strain UW551 with 2 to 6, 40-70 mer probes per gene, with two-fold technical redundancy. The arrays also contain probes for intergenic regions with no technical replicates.
 
Contributor(s) Babujee L, Jacobs JM, Meng F, Allen C
Citation(s) 22807564
Submission date Nov 14, 2011
Last update date Sep 04, 2012
Contact name Lavanya Babujee
E-mail(s) babujee@wisc.edu
Organization name UW-Madison
Street address Department of Plant Pathology
City Madison
ZIP/Postal code 53705
Country USA
 
Platforms (1)
GPL14879 Nimblegen_Ralstonia solanacearum UW551_72k array
Samples (16)
GSM832421 R_solanacearum_UW551_20C_CPG_rep1
GSM832422 R_solanacearum_UW551_20C_CPG_rep2
GSM832423 R_solanacearum_UW551_20C_CPG_rep3
This SubSeries is part of SuperSeries:
GSE33662 Ralstonia solanacearum_GMI1000 and UW551_20 C and 28 C.
Relations
BioProject PRJNA154303

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33661_RAW.tar 39.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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