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Series GSE33999 Query DataSets for GSE33999
Status Public on Dec 01, 2012
Title Genome wide transcriptional portrait of Actinobacillus pleuropneumoniae during invasive disease reveals new strategies for survival and persistence in the host
Organism Actinobacillus pleuropneumoniae
Experiment type Expression profiling by array
Summary The transcriptional profile of the porcine lung pathogen, Actinobacillus pleuropneumoniae, was monitored during the acute phase of infection in its natural host. Bacterial expression profiles of A. pleuropneumoniae isolated from lung lesions of 25 infected pigs were compared in samples taken 6, 12, 24 and 48 hours post infection.
 
Overall design A 84 chip study using total RNA recovered from A. pleuropneumoniae serotype 2 (4226) and serotype 6 (7712640) isolated from infected pig lung tissue during the first 48 of infection. Samples were taken 6, 12, 24 and 48 hours post infection, respectively. Before hybridization the samples were enriched for bacterial RNA and submitted to linear amplification. Each chip measures the expression level of 4,876 target genes from A. pleuropneumoniae with each gene covered by an average of 26.7 probes. Three biological replicates per sample. Microarray data from 3 pigs (no. 39, no. 43 and no. 72) were omitted from the final analysis due to poor signal intensity. A total of 75 microarrays were included in the final analysis.
 
Contributor(s) Klitgaard K, Friis C, Jensen TK, Angen O, Boye M
Citation(s) 22530048
Submission date Nov 29, 2011
Last update date Jan 03, 2013
Contact name Kirstine Klitgaard
E-mail(s) kksc@vet.dtu.dk
Phone 0045 35886265
Organization name National Veterinary Institute, Technical University of Denmark
Street address Bulowsvej 27
City Copenhagen
ZIP/Postal code 1790
Country Denmark
 
Platforms (1)
GPL11003 DTU-VET-Actinobacillus pleuropneumoniae-135K array.v1
Samples (75)
GSM840195 A. pleuropneumoniae__serotype6_6h__rep1 [33-1]
GSM840196 A. pleuropneumoniae__serotype6_6h__rep2 [33-2]
GSM840197 A. pleuropneumoniae__serotype6_6h__rep3 [33-3]
Relations
BioProject PRJNA150573

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33999.txt.gz 1.9 Mb (ftp)(http) TXT
GSE33999_RAW.tar 69.5 Mb (http)(custom) TAR (of XYS)
Processed data included within Sample table
Processed data are available on Series record
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